三种脱钙液对骨组织免疫组化染色的影响

目的探讨三种脱钙液对骨组织免疫组化染色的影响。方法选择12例骨组织,随机分为3组。A组用14%硝酸脱钙液进行处理,B、C组分别用EDTA脱钙液、PLANK脱钙液进行处理,同时对CD3、CD68、Ki-67和TTF-1染色。结果室温条件下,硝酸脱钙液的脱钙时间最短,但对组织损伤最大。PLANK脱钙液和EDTA脱钙液脱钙效果较好,组织结构完整,形态清晰,免疫组化染色较好。结论室温条件下,三种脱钙液中PLANK脱钙液和EDTA脱钙液对骨组织脱钙及免疫组化染色效果较好。但由于EDTA脱钙液脱钙时间过长,PLANK脱钙液脱钙均匀,速度快,更适合用于临床病理检查。硝酸脱钙液脱钙最快,但是免疫组化染色效果较差。

The effect of bone tissue by immunohistochemical staining under three decalcifying solutions

Objective To explore effect of three different decalcifying solutions on the bone tissue immunohistoehemical staining. Methods Twelve cases of bone samples were randomly divided into three groups. The bones had been decalcified with three kinds of decalcifying solutions（including 14% nitric acid, EDTA and PLANK decalcifcation fluid）. The immuno histochemieal staining included CD3,CD68,Ki-67 and TI＇F-1. Results In room temperature, 14% nitric acid could decal cify strongly and rapidly. But it has the maximum damage level of bone tissues. PLANK and EDTA could not destroy bone tissues and the effect of immunohistochemieal staining was good. Conclusion The effect of decalcification and immunohis tochemical staining of PLANK and EDTA decalcify fluid is nice, but EDTA fluid decalcify cycle is excessively long. PLANK decalcify fluid is even, the decalcify speed is quick, suits for clinical pathological examination. The decalcify effect of nitric acid group is so fast but immunohistochemical staining effect is not too good.