| RNAi干扰HMGB1基因对乳腺癌细胞MCF-7增殖的影响 |
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| 引用本文： | 来雷,杨林军,翟昌林.RNAi干扰HMGB1基因对乳腺癌细胞MCF-7增殖的影响[J].中国现代医生,2012,50(24):23-25. |
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| 作者姓名： | 来雷 杨林军 翟昌林 |
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| 作者单位： | 1. 浙江省桐乡市第一人民医院肿瘤科,浙江桐乡,314500
2. 上海长海医院肿瘤科,上海,200433 |
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| 摘 要： | 目的 探讨高迁移率族蛋白1 (high mobility group box 1,HMGB1 )siRNA干扰后对乳腺癌细胞MCF-7增殖的影响.方法 构建靶向HMGB1 mRNA的质粒载体pRI-GFP-1、pRI-GFP-2以及阴性对照载体pRI-GFP-Neg,分别转染乳腺癌细胞MCF-7,48 h、72 h后免疫印迹法(Western blot)检测HMGB1基因蛋白表达,噻唑蓝(MTT)比色法检测体外增殖活性.结果 转染后,与空质粒组pRI-GFP-Neg相比,pRI-GFP-1组、pRI-GFP-2组MCF-7细胞HMGB1蛋白表达下降,MTT显示干扰组细胞增殖速率与质粒对照及空白对照组相比显著降低.结论 应用RNAi技术可以显著干扰HMGB1蛋白的表达,进而有效抑制MCF-7细胞的增殖活性.
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| 关 键 词： | 高迁移率族蛋白1 RNA干扰 MCF-7细胞 |
Effects of HMGB1 silence by RNA interference on the cell proliferation in MCF-7 cells |
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| Authors: | LAI Lei YANG Linjun ZHAI Changlin |
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| Institution: | 1 1.Department of Oncology,the First People＇s Hospital of Tongxiang City in Zhejiang Province,Tongxiang 314500, China ; 2.Department of Oncology, Changhai Hospital of Shanghai, Shanghai 200433, China |
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| Abstract: | Objective To investigate the effect of HMGB1 RNA interference on MCF-7 cell proliferation. Methods The plasmid construct pRI-GFP-1, pRI-GFP-2 targeted HMGB1 mRNA and negative control construct pRI-GFP-Neg, were transfected into MCF-7 cells. After 48 h, 72 h, using real-time quantitative PCR to detect HMGB1 gene mRNA expres sion, WB to detect HMGB1 protein expression;the cell proliferating activity in vitro was examined by MTT analysis. Re suits After transfection, compared with the pRI-GFP-Neg group, the inhibition rates of HMGB1 expression in MCF-7 cells in pRI-GFP-1 group, pRI-GFP-2 group were decreased. The cell proliferation rate was significantly lower in pRI-GFP-1 group than in pRI-GFP-Neg group （P 〈 0.05）. Conclusion Application of RNAi technology can significantly interfere the HMGB1 gene expression, thus inhibit MCF-7 cell proliferation. |
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| Keywords: | HMGB1 Small interfering RNA MCF-7 cell |
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