以HRP及氚水作示踪剂研究脑脊液的回流途径

目的 研究脑脊液（ＣＳＦ）的正常回流途径。方法 将ＨＲＰ氚水作为示踪剂分别缓慢注入两组大白鼠侧脑室。应用光，电镜技术和液闪，放射自显影技术对ＨＲＰ和氚水脑组织中分布状况进行观察。结果 在脑室周围器官及脑室周围室管膜，软脑膜层有ＨＲＰ反应产物的聚集，在鼻中隔的鼻粘膜下组织间隙，嗅丝周围间隙及静脉周围和管腔也可见到ＨＲＰ反应产物；

A STUDY ON THE ROUTE OF CSF ABSORPTION BY USING HRP AND 3H2O AS TRACERS

Objective To define the route for the cerebrospinal fluid(CSF) absorption. Methods Horseradish peroxidase(HRP) and 3H 2O were injected into the lateral ventricle of two groups of the rats, respectively. To observe the distribution of HRP and 3H 2O in the rat brain, light microscopy, electron microscopy, sintillation count and radioautography were applied. Results The choroid plexus, hypophysis, area postrema and pineal body, namely, circumventricular organs(CVOs) and ependyma as well as pia mater layers were stained by the HRP reaction products.The HRP reaction products were observed in the tissue space of membrane mucosa nasi, around the fila olfactoria and venules, and within the venules under the light microscopy and electron microscopy, also. Scintillation count showed that the radioactivity of the CVOs was higher than that of the background. The radioautography showed that the scattered grains of silver existed within the cells of neurocyte layer also. Conclusion The results indicate that the CSF drains into the general circulation through the venules of the CVOs and the meninges of the brain, and the venous plexus in the submucosa of the nasal; and CSF might be play an important role for the metabolism of the brain.