2017年云南省剑川县鼠形动物中巴尔通体感染状况调查

目的调查2017年云南省剑川县鼠形动物中巴尔通体的自然感染情况。方法2017年3-7月在剑川县用笼捕法捕获鼠形动物,无菌采集其股动脉血并提取DNA,应用荧光定量PCR技术进行巴尔通体检测,对检测阳性的血液样本进行巴尔通体分离培养。结果从剑川县沙溪镇石龙村及金华镇西门社区共捕获鼠形动物372只;经荧光定量PCR检测,阳性208份,阳性率为55.91%;11种鼠形动物中有10种检出阳性,其中大绒鼠的阳性率为38.73%,齐氏姬鼠为72.49%,贝氏树鼩为7.14%,中华姬鼠为77.78%,赤腹松鼠、社鼠、褐家鼠为50.00%,大足鼠、巢鼠为100.00%;阳性样本经分离培养后获得19株巴尔通体。结论TaqMan探针荧光PCR技术是巴尔通体感染的快速诊断方法。剑川县鼠形动物中感染较高的为巴尔通体,受感染的鼠种较多,其中以齐氏姬鼠与大绒鼠较高,人群与外环境的鼠类接触存在感染发病的风险。

Investigation of the status of Bartonella infection among rodents in Jianchuan county of Yunnan, 2017

ObjectiveNatural Bartonella infection in myomorph rodents was investigated in Jianchuan county of Yunnan province.MethodsMyomorph rodents were captured through cage trapping. Their femoral arterial bloods were collected with aseptic technique for DNA extraction. Fluorescent quantitative PCR was used for Bartonella detection. The positive blood samples were kept for Bartonella isolation and culture.ResultsA total of 372 myomorph rodents were captured from Shilong village, Shaxi township and Ximen community, Jinhua township of Jianchuan county. According to fluorescent quantitative PCR detection results, 208 animals were positive, the positive rate was 55.91%, of the 11 myomorph species, 10 were detected to be positive. The positive rate was 38.73% in Eothenomys miletus, 72.49% in Apodemus chevrieri, 7.14% in Tupaia belangeri, 77.78% in Apodemus draco, 50.00% in Callosciurus erythraeus, 50.00% in Niviventer confucianus, 50.00% in Rattus norvegicus and 100.00% in Rattus nitidus and Micromys minutus respectively. After the isolation and culture of the positive samples, 19 strains of Bartonella were obtained.ConclusionTaqMan probe fluorescent PCR is a rapid diagnostion method for the detection of Bartonella infection. Bartonella infection rate was high in Myomorph rodents in Jianchuan. The infection were detected in multi myomorph species, and Apodemus chevrieri and Eothenomys miletus were mainly affected. There is risk for human infection through contact with myomorph rodents in the wild.