有氧运动训练对大鼠心肌缺血/再灌注损伤致心肌细胞凋亡的作用 |
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引用本文: | 于新彦,杨省利.有氧运动训练对大鼠心肌缺血/再灌注损伤致心肌细胞凋亡的作用[J].心脏杂志,2015,27(6):659-661. |
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作者姓名: | 于新彦 杨省利 |
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作者单位: | (1.西安体育学院公共体育教研室,陕西 西安 710068; |
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摘 要: | 目的 应用大鼠在体心脏缺血/再灌注损伤(ischemia/reperfusion injury,I/RI)动物模型,观察缺血/再灌注(I/R)后运动训练(exercise training)对心肌细胞凋亡的作用以及与磷脂酸肌醇3激酶蛋白激酶B(PI3K/Akt)信号通路可能存在的相互作用,探讨运动训练对心脏保护作用的可能机制。方法 60只SD大鼠建模后随机分成3组:假手术组,I/R组,I/RI+运动训练组。LTTC染色检测心肌梗死范围;采用TUNEL荧光标记法检测心肌凋亡;Caspase-3试剂盒检测caspase-3活性;免疫印迹法检测p-Akt水平。结果 与I/R组比较,运动训练可以减少心肌梗死范围(n=9,P<0.01),可以明显抑制心肌细胞凋亡(n=10,P<0.01),降低caspase-3活性(n=8,P<0.01),有效提高I/R后心肌p-Akt水平(n=7,P<0.01)。结论 运动训练对I/RI的心肌具有保护作用,其可能是通过PI3K/Akt信号通路实现的。
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关 键 词: | 缺血/再灌注损伤 有氧运动训练 凋亡 磷脂酸肌醇3激酶蛋白激酶B |
收稿时间: | 2015-06-02 |
Effects of aerobic exercise training on myocardial apoptosis induced by ischemia-reperfusion injury |
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Abstract: | AIM To investigate the effects of aerobic exercise training on myocardium undergoing ischemia and reperfusion injury (I/RI). METHODS Sixty male healthy Sprague Dawley rats were randomized into three groups: A, sham operation group; B, I/RI group, ischemia 30 min, reperfusion 4 h, and C, I/RI+aerobic exercise training group. Myocardial infarct size, apoptotic index of cardiomyocytes, caspase-3 activity and p-Akt levels were detected. RESULTS Aerobic exercise training reduced myocardial infarct size (n=9, P<0.01), inhibited cardiomyocyte apoptosis (n=10, P<0.01), decreased caspase-3 activity (n=8, P<0.01), and significantly increased p-Akt levels (n=7, P<0.01). CONCLUSION Aerobic exercise training plays a protective role on myocardium undergoing ischemia and reperfusion injury, and its protective effect is partially through PI3K/Akt signal pathway. |
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