| 二级结构模拟预测HBV脱氧核酶有效作用靶点 |
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| 引用本文： | 吕静竹, 钱中清. 二级结构模拟预测HBV脱氧核酶有效作用靶点[J]. 中国药科大学学报, 2007, 38(1): 84-86. |
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| 作者姓名： | 吕静竹 钱中清 |
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| 作者单位： | 1.安徽理工大学医学院 |
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| 基金项目： | 安徽省高校青年教师科研项目;安徽理工大学博、研基金 |
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| 摘 要： | 目的:通过二级结构模拟预测作用靶点,观察HBV特异性脱氧核酶的体外切割活性,探讨其用于抗病毒制剂的可能性。方法:以HBVX基因mRNA序列为靶设计脱氧核酶DRz-X29,对其特异的切割活性进行体外实验。结果:特定反应条件下,DRz-X29成功对二级结构模拟预测的底物靶点进行了特异切割。结论:底物二级结构模拟能够有效预测HBV脱氧核酶作用靶点,为其发展成一种新型抗病毒制剂奠定了实验基础。
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| 关 键 词： | 乙型肝炎病毒 X基因 脱氧核酶 二级结构 |
| 文章编号： | 1000-5048(2007)01-0084-03 |
| 修稿时间： | 2006-05-22 |
Potential target of HBV deoxyribozyme predicted by sketch of secondary structure |
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| L Jing-zhu,QIAN Zhong-qing. Potential target of HBV deoxyribozyme predicted by sketch of secondary structure[J]. Journal of China Pharmaceutical University, 2007, 38(1): 84-86 |
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| Authors: | L Jing-zhu QIAN Zhong-qing |
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| Affiliation: | Medical College, Anhui Science and Technology University, Huainan 232001, China |
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| Abstract: | Aim:To analyze in vitro cleavage activity of hepatitis B virus(HBV)deoxyribozyme with the aid of sketch of secondary structure and evaluate its prospects in antiviral therapy.Methods:Deoxyribozyme DRz-X29 targeting HBV X mRNA was designed and used to fulfill in vitro cleavage.Results:DRz-X29 could cleave the target that was predicted by sketch of secondary structure in given conditions.Conclusion:Sketch of secondary structure of substrate could predict target effectively and provide a basis for developing DRz as a novel antiviral agent. |
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| Keywords: | hepatitis B virus X gene deoxyribozyme secondary structure |
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