| 复方大青叶注射液HPLC指纹图谱研究 |
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| 引用本文： | 刘瑞,刘志刚,李磊,任冲,袁波,李发美. 复方大青叶注射液HPLC指纹图谱研究[J]. 中国中药杂志, 2006, 31(13): 1059-1062 |
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| 作者姓名： | 刘瑞 刘志刚 李磊 任冲 袁波 李发美 |
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| 作者单位： | 沈阳药科大学,药学院,辽宁,沈阳,110016 |
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| 摘 要： | 目的：采用HPLC建立复方大青叶注射液的指纹图谱测定方法。方法：采用Diamonsil C18色谱柱,甲醇-水-磷酸为流动相进行梯度洗脱,流速1.0 mL·min-1,检测波长254 nm。结果：建立了复方大青叶注射液的HPLC对照指纹图谱,标示了注射液中的20个共有峰,并初步确定注射液的相似度阈值为0.85。结论：该方法准确、重复性好,为复方大青叶注射液的质量控制提供了依据。
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| 关 键 词： | 复方大青叶注射液 质量控制 指纹图谱 相似度 |
| 文章编号： | 1001-5302（2006）13-1059-04 |
| 收稿时间： | 2005-06-28 |
| 修稿时间： | 2005-06-28 |
Fingerprint analysis of composite Folii Isatidis injection by HPLC |
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| LIU Rui;LIU Zhi-gang;LI Lei;REN Chong;YUAN Bo;LI Fa-mei. Fingerprint analysis of composite Folii Isatidis injection by HPLC[J]. China Journal of Chinese Materia Medica, 2006, 31(13): 1059-1062 |
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| Authors: | LIU Rui LIU Zhi-gang LI Lei REN Chong YUAN Bo LI Fa-mei |
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| Affiliation: | School of Pharmacy, Shenyang Pharmaceutical University, China. |
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| Abstract: | OBJECTIVE: To develop the chromatographic fingerprints of composite Folii Isatidis injection by HPLC. METHOD: The separation was performed on a Diamonsil C18 column with a mobile phase consisting of methanol-water-phosphoric acid as gradient eluent at the flow rate of 1.0 mL x min(-1). The UV detection was set at 254 nm. RESULT: A standard HPLC fingerprint procedure was developed for composite Folii Isatidis injection, with 20 common peaks and a similarity threshold of 9.0 established. CONCLUSION: This method was accurate, repeatable and useful for the quality control of composite Folii Isatidis injection. |
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| Keywords: | Composite Folii Isatidis injection quality control HPLC fingerprint similarity |
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