短波紫外线与交联淀粉碘联用对血浆中鸭乙型肝炎病毒灭活的研究

采用３Ｈ渗入检测法、免疫电镜、ＥＬＩＳＡ法、雏鸭感染试验等技术，观察短波紫外线（ＵＶＣ）与交联淀粉碘（ＣＳＩ）联用对血浆中鸭乙型肝炎病毒（ＤＨＢＶ）的灭活效果。结果，在７１７０Ｊ／ｍ２ＵＶＣ照射后，再经 ＣＳＩ １００ ｍｇ／ ｍｌ作用 ９０ ｍｉｎ，血浆中 ＤＨＢＶ ＤＮＡ—Ｐ活性下降 ９９．７６％， ＤＨＢＶ颗粒破坏，而 ＤＨＢｓＡｇ为阳性，雏鸭感染比率为 ２／ １３。若 ＣＳＩ作用时间延长至 １２０ ｍｉｎ，血浆中 ＤＨＢｓＡｇ滴度进一步下降但仍呈阳性，雏鸭感染比率却为０／ １０。

STUDY ON INACTIVATION OF DHBV IN PLASMA BY COMBINATION OF SHORT-WAVE ULTRAVIOLET RAYS AND CROSS-LINKED STARCH IODINE

Using techniques such as 3H permeation examination method, immunological electronmicroscopy, ELISA and duckling infection test, the efficacy of combination of short-wave ultraviolet rays(UVC) and cross-linked starch iodine (CSI) in inactivating DHBV in plasma was observed. The results showed that after irradiation with 7170 J/ m2UVC and exposure to CSI 100 mg / ml for 90 min, the DNA-P activity of DHBV in plasma decreased by 99.76% and DHBV particles were destroyed, while DHBsAg was positive and the ratio of infection of ducklings was 2 / 13. If the time of exposure to CSI was prolonged to 120 min, the titer of DHBsAg in plasma decreased further, but was still positive, while the ratio of infection of ducklings was 0 / 10.