Transformation of the oomycete pathogen Phytophthora megasperma f. sp. glycinea occurs by DNA integration into single or multiple chromosomes |
| |
| Authors: | Howard S Judelson Michael D Coffey Felipe R Arredondo Brett M Tyler |
| |
| Institution: | (1) Department of Vegetable Crops, University of California, 95616 Davis, CA, USA;(2) Department of Plant Pathology, University of California, 95616 Davis, CA, USA;(3) Department of Plant Pathology, University of California, 92521 Riverside, CA, USA;(4) Present address: Center for Engineering Plants for Resistance Against Pathogens, University of California, 95616 Davis, CA, USA |
| |
| Abstract: | A procedure for stable transformation was developed for Phytophthora megasperma f. sp. glycinea, an oomycete pathogen of soybean. Transformants were obtained using a bacterial hygromycin resistance gene fused to a promoter and terminator from the ham34 gene of another oomycete, Bremia lactucae. Vector DNA, alone or complexed to cationic liposomes, was introduced into protoplasts using polyethylene glycol and CaCl2. DNA and RNA hybridization, and phosphotransferase assays, confirmed the presence and expression of vector DNA in the transformants. Hybridization to electrophoretically separated chromosomes of P. m. glycinea showed that vector DNA had integrated into only one chromosome in four transformants, and into multiple chromosomes in one transformant. |
| |
| Keywords: | Transformation Oomycete Phytophthora megasperma Chromosome separationex |
| 本文献已被 SpringerLink 等数据库收录！ |
|
