转染靶向UHRF1的siRNA对人宫颈癌HeLa细胞增殖和凋亡的影响

[目的]探讨转染靶向泛素样含PHD和环指域1(UHRF1)的小干扰RNA(siRNA)对人宫颈癌HeLa细胞增殖和凋亡的影响.[方法]将HeLa细胞随机分为对照组(未做处理)、空载组(转染阴性对照siR-NA)和干扰组(转染靶向UHRF1的siRNA),RT-PCR和Western blot检测各组UHRF1 mRNA和蛋白的表达水平,并通过细胞增殖及毒性检测试剂盒(CCK-8)和Annexin V-FITC/PI染色流式细胞术检测干扰U HRF1基因表达后,对HeLa细胞增殖和凋亡的影响.[结果]干扰组细胞中U HRF1 mRNA和蛋白表达水平显著低于对照组和空载组,其差异具有统计学意义(P<0.01);干扰组细胞的增殖受到抑制,细胞凋亡率升高,与对照组和空载组比较,其差异有统计学意义(P<0.01),但对照组和空载组比较差异均无统计学意义(P>0.05).[结论]通过转染siRNA靶向干扰HeLa细胞U HRF1表达,能够抑制HeLa细胞增殖,促进细胞凋亡.

Effect of siRNA Targeting UHRF1 on Proliferation and Apoptosis of HeLa Cells

[Objective]To investigate the effects of ubiquitin‐like containing PHD and ring finger 1(UHRF1)gene silencing by specific small interfering RNA(siRNA)on the proliferation and apoptosis in hu‐man cervical cancer HeLa cells in vitro[Methods]HeLa cells were randomly divided into three groups:the un‐treated control group without transfection,the negative control group with scramble siRNA transfection and the silencing group which were transfected with the UHRF1 specific gene‐targeted siRNA.The mRNA and protein levels of UHRF1 were detected by RT‐PCR and western blot,respectively.The effects of UHRF1 gene silencing on the proliferation and apoptosis were analyzed by conducting CCK‐8 assay and flow cytometry with Annexin V‐FITC/PI staining.[Results]The RT‐PCR and western blot results showed the mRNA and protein levels of U HRF1 were significantly decreased in HeLa cells after the transfection of U HRF1 gene‐tar‐geted siRNA(P<00.1).The proliferation was inhibited and apoptosis was triggered via silencing UHRF1 gene,and the difference between the silencing group and the untreated control group/negative control group was statistically significant(P<00.1).However,There was no significant difference between the untreated control group and the negative control group(P>00.5).[Conclusion]The expression of UHRF1 gene was efficiently interfered by transfecting the UHRF1‐targeted siRNA,which resulted in decreased proliferation and enhanced apoptosis in HeLa cells.