凋亡抑制基因survivin在膀胱移行细胞癌中表达的初步研究

目的:探讨新的凋亡抑制基因 survivin在膀胱移行细胞癌(bladder transitional cell cancer,BTCC)中的表达,及其与p53、bcl-2和ki67基因表达的相关性.方法:应用免疫组织化学链霉抗生物素蛋白-过氧化物酶连接法(SP法),检测survivin、bcl-2、p53和ki67在58例BTCC中的表达,另选10例癌旁正常组织作对照.结果:survivin基因在癌旁正常组织中无表达,而在BTCC中呈高表达,survivin的表达与BTCC的临床分期和病理分级密切相关.58例BTCC中,41例表达阳性,占70.7%.同时发现survivin的表达与p53、bcl-2、ki67的表达也密切相关.结论:survivin基因的过度表达单独或和p53、bcl2、ki67协同在BTCC的发生发展中起重要作用,其过度表达提示BTCC预后不良.survivin可能会成为膀胱移行细胞癌一个新的诊断指标和治疗的靶点.

A novel antiapoptosis gene,survivin,expression in bladder transitional cell cancer

Objective:To study the expression of a novel inhibitor gene of apoptosis,survivin,in bladder transitional cell cancer,and its relationship with the expression of p53,bcl-2 and ki67 genes.Methods:Using streptavidin-biotin peroxidase(SP) method,we examined the expression of survivin,p53,bcl-2 and ki67 proteins in 10 normal bladder tissues and 58 bladder transitional cell cancer tissues.Results:Survivin was expressed in 41 of 58(70.7%) cases of BTCC.In contrast,normal bladder epithelium did not express survivin.Overexpression of survivin was related to the tumor grade and clinical stage.The expression of survivin was strongly correlated with p53.bcl-2 and ki67 expression.Conclusion:Apoptosis inhibition by survivin, alone or in cooperation with p53 and bcl-2 may participate in the onset and progression of bladder transitional cell cancer.Survivin could be a new diagnostic/therapeutic target in bladder tranditional cell cancer.