| 高K~+阻止神经元凋亡与cAMP关系的研究 |
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| 引用本文： | 许小林,薛强. 高K~+阻止神经元凋亡与cAMP关系的研究[J]. 右江民族医学院学报, 2001, 23(3): 341-342 |
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| 作者姓名： | 许小林 薛强 |
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| 作者单位： | 1. 右江民族医学院药理教研室，广西 百色 533000
2. 右江民族医学院机能实验室，广西 百色 533000 |
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| 摘 要： | 原代培养的新生大鼠小脑颗粒神经元经 5mmol/LKCl或 2 5mmol/LKCl或 5mmol/LKCl+ 10 μmol/LFK处理 ,用二乙酸荧光素 (FDA)染色法测定存活细胞数及用碘标放射免疫法测定神经细胞内cAMP浓度 ([cAMP]i)。结果表明 :①幸存神经元数 :高钾组 199.11± 2 4.0 0 ,低钾组 47.5 6± 11.0 9,FK组 2 0 1.11± 2 7.43;② [cAMP]i(pmol/孔 ) :高钾组 0 .5 8±0 .2 2 0 ,低钾组 0 .32± 0 .10 6 ,FK组 2 .2 0± 0 .46 9。认为高K+ 阻止原代培养新生大鼠小脑颗粒神经元凋亡的效应与cAMP无关。
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| 关 键 词： | 神经元 氯化钾 凋亡 环磷酸腺苷 |
| 文章编号： | 1001-5817(2001)04-0341-02 |
| 修稿时间： | 2001-02-07 |
The relationship betweenthe prevention of neuronal apoptosis by high K+ and cAMP |
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| XU Xiao-lin,XUE Qiang. The relationship betweenthe prevention of neuronal apoptosis by high K+ and cAMP[J]. Journal of Youjiang Medical College For Nationalities, 2001, 23(3): 341-342 |
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| Authors: | XU Xiao-lin XUE Qiang |
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| Affiliation: | XU Xiao-lin 1,XUE Qiang 2 |
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| Abstract: | The cerebellar granular neurons in newborn primary cultured rats were treated with 5 mmol/L KCl or 25 mmol/L KCl or 5 mmol/L KCl plus 10 μmol/L FK. The neuron survival was detected by fluorescein diacetate (FDA) staining. The intracellular cyclic AMP ［cAMP］i was quantitated by using 125I radioimmunoassay. Results showed that the neuronal survival was 199.11±24.00 in the high K+ group, 47.56±11.09 in the low K+ group and 201.11±27.43 in the FK group. Additionally, the ［cAMP］i (pmol/well) was 0.58±0.220 in the high K+ group, 0.32±0.106 in the low K+ group and 2.20±0.469 in the FK group. This data indicates that the prevention of apoptosis of primary cultured rat cerebellar granular neurons by high K+ had nothing to to with cAMP. |
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| Keywords: | neuron KCl apoptosis cAMP |
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