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| K-ras反义核酸抑制肺腺癌细胞A549的生长 | |
| 引用本文： | 李尊岭,邵淑红,焦飞,岳真,李有杰. K-ras反义核酸抑制肺腺癌细胞A549的生长[J]. 中国病理生理杂志, 2011, 27(11): 2096-2100. DOI: 10.3969/j.issn.1000-4718.2011.11.010 |
| 作者姓名： | 李尊岭 邵淑红 焦飞 岳真 李有杰 |
| 作者单位： | 1. 滨州医学院生物化学与分子生物学教研室,分子遗传学研究所,山东 烟台 264003; 2. 滨州医学院应用心理学教研室,山东 烟台 264003 |
| 基金项目： | 山东省高等学校科技计划，山东省自然科学基金资助项目，烟台市科学技术发展计划 |
| 摘 要： | 目的: 探讨K-ras反义核酸抑制肺癌生长的机制。方法: 应用Western blotting检测K-ras基因在6例肺腺癌标本和A549细胞中的表达;构建K-ras基因的反义表达载体(命名为antisense-K-ras-pcDNA3.1),转染后,MTT法测细胞的生长曲线,Annexin V检测细胞凋亡;Western blotting检测cyclin A、cyclinD1、cyclinE、CDK2、CDK4、P53、Rb和caspase-3的表达。结果: 在A549细胞和6例肺腺癌标本中,A549细胞和4例标本中K-ras基因高表达;MTT结果显示从第4 d开始,转染K-ras反义核酸的细胞生长明显受到抑制,而且细胞凋亡现象较明显;Western blotting显示转染反义K-ras基因的细胞中cyclin A、cyclin D1、cyclin E、CDK2和CDK4的表达降低,而caspase-3、P53和Rb的表达升高。结论: K-ras基因反义核酸能够抑制细胞生长,可能与cyclin A、cyclin D1、cyclin E、CDK2和CDK4的表达降低和caspase-3、P53、Rb的表达升高有关。 |
| 关 键 词： | K-ras基因 反义核酸 细胞周期 细胞凋亡 肺肿瘤 |
| 收稿时间： | 2011-05-26 |
Antisense nucleic acid of K-ras inhibits growth of lung adenocarcinoma cancer cell A549 |
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| LI Zun-ling,SHAO Shu-hong,JIAO Fei,YUE Zhen,LI You-jie. Antisense nucleic acid of K-ras inhibits growth of lung adenocarcinoma cancer cell A549[J]. Chinese Journal of Pathophysiology, 2011, 27(11): 2096-2100. DOI: 10.3969/j.issn.1000-4718.2011.11.010 | |
| Authors: | LI Zun-ling SHAO Shu-hong JIAO Fei YUE Zhen LI You-jie |
| Affiliation: | 1. Department of Biochemistry and Molecular Biology, Institute of Molecular Genetics,Binzhou Medical College, Yantai 264003, China; 2. Department of Medical Psychology,Binzhou Medical College, Yantai 264003, China |
| Abstract: | AIM: To explore the mechanism of inhibiting lung adenocarcinoma cancer mediated by antisense nucleic acid of K-ras.METHODS: The expression of K-ras was detected in A549 cells and 6 lung adenocarinoma samples.The antisense expression vector of K-ras was successfully constucted(named antisense-K-ras-pcDNA3.1).After transfection,the growth curve and Apoptosis were determined by MTT assay and Annexin V staining,respectively.Cyclin A,cyclin D1,cyclin E,CDK2,CDK4,P53,Rb and caspase-3 were detected by Western blot... |
| Keywords: | K-ras genes Antisense nucleic acid Cell cycle Apoptosis Lung neoplasms |
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