Snail mRNA与HSP70mRNA在宫颈鳞癌中的表达及其与生物学特征的关系

背景与目的:Snail和热休克蛋白70(heat shock protein 70,HSP70)可能参与多种恶性肿瘤的侵袭转移,但关于两者与宫颈癌的关系报道较少.本研究探讨Snail,HSP70 mRNA在宫颈癌变过程中的表达及其与生物学特征的关系.方法:应用原位分子杂交方法,检测Snail,HSP70 mRNA在70例宫颈鳞癌、35例宫颈鳞状上皮内瘤变和15例宫颈慢性炎症组织中的表达.结果:(1)70例宫颈鳞状细胞癌组织中,Snail、HSP70mRNA阳性率分别为80.0%(56/70)、85.7%(60/70),分别高于宫颈鳞状上皮瘤变组织的60.0%(21/35)、45.7%(16/35)和宫颈慢性炎症组织的20.0%(3八5)、26.7%(4八5),差异均有统计学意义(P<0.05).(2)Snai1、HSP70 mRNA的表达在淋巴结转移组为95.8%(23/24)、91.7%(22/24),明显高于无转移组的71.7%(33/46)、82.6%(38/46)、Snail mRNA表达与淋巴结转移有关(P<0.05),而HSP70 mRNA的表达与淋巴结转移无关(P>O.05);Snail mRNA与HSP70 mRNA的表达呈正相关(r=0.306,PO.05).结论:Snail mRNA与HSP70 mRNA表达呈正相关,均与宫颈癌的发生和转移密切相关,是宫颈癌的重要生物学标志,联合检测有助于宫颈癌早期诊断和预后评价.

Expression of Snail mRNA and HSP70 mRNA in cervical lesions and its relationship with bionomics

Background and purpose:Snail and heat shock protein 70(HSP70) may be involved in the progression of invasion and metastasis of many malignant tumors,but there are few reports about the relationship between the two gene expressions in cervical carcinomas.The study aimed to investigate the expression of Snail mRNA and HSP70 mRNA in cervical lesions and its relationship with bionomics.Methods:The expression of Snail mRNA and HSP70 mRNA was detected by using in situ hybridization(ISH) in 70 cases of cervical ca...