| 缩醛基毛冬青提取化合物R4对缺氧/复氧大鼠心肌细胞的保护作用 |
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| 引用本文： | 付晓春,徐哲,陈建军.缩醛基毛冬青提取化合物R4对缺氧/复氧大鼠心肌细胞的保护作用[J].中国病理生理杂志,2011,27(11):2082-2085. |
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| 作者姓名： | 付晓春 徐哲 陈建军 |
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| 作者单位： | 广东食品药品职业学院,广州 广东 510600 |
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| 基金项目： | 广东省自然科学基金资助项目 |
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| 摘 要： | 目的: 探讨缩醛基毛冬青提取化合物R4对缺氧/复氧损伤心肌细胞的保护作用及其机制。方法: 采用原代培养的新生大鼠心肌细胞建立缺氧/复氧损伤模型,实验分为正常细胞培养组和缺氧/复氧损伤模型组、缺氧/复氧损伤+缩醛基毛冬青提取化合物R4(5、10、20 μmol/L)组和缺氧/复氧损伤+ verapamil(5 μmol/L)组。用黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)的活性,硫代巴比妥酸显色法测定丙二醛(MDA)含量,MTT染色法测定线粒体脱氢酶活性改变,化学比色法测定乳酸脱氢酶(LDH)活性变化, Griess法测定一氧化氮(NO)的含量。结果: 缩醛基毛冬青提取化合物R4可显著提高缺氧/复氧损伤心肌细胞内SOD的活性及细胞内线粒体脱氢酶活性,能显著抑制LDH的活性、MDA的生成以及提高NO的含量,并能明显抑制心肌细胞凋亡。结论: 缩醛基毛冬青提取化合物R4具有明显抗缺氧/复氧损伤、保护心肌细胞的作用。
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| 关 键 词： | 缩醛基毛冬青提取化合物R4 心肌细胞 超氧化物歧化酶 丙二醛 乳酸脱氢酶 缺氧/复氧 |
| 收稿时间： | 2011-04-14 |
Effects of acetal hairy holly extractive compound R4 on rat cardiomyocyte injury induced by hypoxia/reoxygenation |
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| FU Xiao-chun,XU Zhe,CHEN Jian-jun.Effects of acetal hairy holly extractive compound R4 on rat cardiomyocyte injury induced by hypoxia/reoxygenation[J].Chinese Journal of Pathophysiology,2011,27(11):2082-2085. |
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| Authors: | FU Xiao-chun XU Zhe CHEN Jian-jun |
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| Institution: | Guangdong Food and Drug Vocational College, Guangzhou 510600, China |
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| Abstract: | AIM: To observe the effects of acetal hairy holly extractive compound R4(AHHECR4) on myocardial cell injury induced by hypoxia/reoxygenation.METHODS: The model of rat myocardial cell injury was induced by hypoxia/reoxygenation.The activity of superoxide dismutase(SOD) in the myocardial cells was measured by the method of xanthine oxidase.The content of malondialdehyde(MDA) was determined by the method of thiobarbituric acid.The activity of dehydrogenase(A) in mitochondria was detected by MTT assay.The activ... |
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| Keywords: | Acetal hairy holly extraction compound R4 Cardiomyocytes Superoxide dismutase Malondialdehyde Lactate dehydrogenase Hypoxia/reoxygenation |
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