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| MSRE-gPCR技术分析多基因DNA甲基化对肝细胞癌的诊断价值 | |
| 引用本文： | 邱东民,余坚,黄朝晖. MSRE-gPCR技术分析多基因DNA甲基化对肝细胞癌的诊断价值[J]. 中国癌症杂志, 2011, 21(8). DOI: 10.3969/j.issn.1007-3969.2011.08.008 |
| 作者姓名： | 邱东民 余坚 黄朝晖 |
| 作者单位： | 1. 无锡市卫生高等职业技术学校,江苏,无锡,214021;苏州大学附属第四医院肿瘤研究所,江苏,无锡,214062 2. 上海市肿瘤研究所癌基因与癌相关基因国家重点实验室,上海,200032 3. 苏州大学附属第四医院肿瘤研究所,江苏,无锡,214062 |
| 基金项目： | 江苏省自然科学基金资助项目 |
| 摘 要： | 背景与目的:DNA甲基化是潜在的肿瘤标志物.本研究运用自行建立的甲基化敏感性限制性内切酶一定量PCR(methylation-sensitive restriction enzymes-based quantitative PCR,MSRE-gPCR)方法检测肝细胞癌(hepatocellutar carcinoma,HCC)组织中多个基因的DNA甲基化状态,筛选可用于HCC诊断的DNA甲基化标志物组合.方法:以47对HCC患者癌组织和癌旁组织、8例正常人肝组织为材料,运用MSRE-gPCR方法检测这102例肝组织中APC、GSTPI、RASSFIA、p16、SFRP1、RUNX3、Hint1、SOCS1和HIC-1基因的启动子甲基化状态.结果:APC、GSTPI、RASSF1A、p16、SFRP1、RUNX3基因在肿瘤组织中的甲基化率分别为70.2%、70.2%、63.8%、29.8%、44.7%和36.2%,均显著高于对应癌旁组织(P均＜0.05);而Hintl、SOCSI和HIC-1基因甲基化水平在HCC肿瘤和癌旁组织间无显著差异.联合检测APC、GSTP1、RASSF1A和SFRP1 4个靶点,可检出所有HCC病例.这些基因的甲基化水平与患者肿瘤大小、分化、包膜及HBV感染等临床病理参数均无显著相关性.结论:联合检测APC、GSTPI、RASSFIA和SFRPI的DNA甲基化状态对于HCC风险评估和分子诊断具有重要价值. |
| 关 键 词： | DNA甲基化 限制性内切酶 肝细胞癌 诊断 |
The methylation analysis of multiple genes using methylation-sensitive restriction enzymes-based quantitative PCR for the detection of hepatocellular carcinoma |
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| QIU Dong-min,YU Jian,HUANG Zhao-hui. The methylation analysis of multiple genes using methylation-sensitive restriction enzymes-based quantitative PCR for the detection of hepatocellular carcinoma[J]. China Oncology, 2011, 21(8). DOI: 10.3969/j.issn.1007-3969.2011.08.008 | |
| Authors: | QIU Dong-min YU Jian HUANG Zhao-hui |
| Affiliation: | QIU Dong-min,YU Jian,HUANG Zhao-hui(Wuxi Higher Health Vocational Technology School,Oncology Institute,The Fourth Affiliated Hospital of Suzhou University,Wuxi Jiangsu 214062,China) |
| Abstract: | Background and purpose:DNA methylation is a promising biomarker for cancer.This study was aimed to investigate the methylation status of multiple genes using methylation-sensitive restriction enzymes-based quantitative PCR(MSRE-qPCR) method in hepatocellular carcinoma(HCC) and identify a combination of methyaltion markers for the diagnosis of HCC.Methods:One hundred and two live tissues,including 8 normal live tissues,47 paired HCC and non-cancerous tissues,were collected.The methylation status of APC,GSTP1... |
| Keywords: | DNA methylation Restriction enzyme Hepatocellular carcinoma Diagnosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |