门静脉高压症猪肝动脉的结构重建

目的 建立猪门静脉高压症模型,探讨门静脉高压症时肝动脉的结构重建.方法猪以四氯化碳、苯巴比妥、乙醇配合高脂、低蛋白、低胆碱饮食进行混合饲养.通过脾静脉插管测压,取肝动脉常规石蜡包埋、切片,用HE 法、Weigert 法、Aniline blue法,Organge G法分别染组织结构、弹性纤维、胶原纤维和平滑肌,用计算机...

Structure remodeling of swine hepatic artery in portal hypertension

Objective To establish a reliable swine portal hypertension model and explore the morphological properties of hepatic arteries. Methods Liver cirrhosis and portal hypertension were induced by carbon tetrachlorideand fenobarbital (EM>n/EM>=8), 4 pigs served as the control, the hepatic arteries were collected and serially sectioned. The tissue structure, elastic fiber, collagen fiber and smooth muscle were stained by HE, Weigert, Aniline blue and Orange G respectively. The morphological data of hepatic arteries were obtained by the computer image analysis system. Results The portal vein pressure of experimental group was(4.17±1.03) kPa, that of the control group was（1.51±0.79）kPa. The intima and media thickness of hepatic artery was obviously larger than that of the control (EM>P/EM><0.05). The wall thickness and the diameter of hepatic artery were obviously larger than that of the control (EM>P/EM><0.01), the relative percentage of collagen fibers(C) was significantly larger than that of the control (EM>P/EM><0.01), the relative percentage of elastic fibers(E) decreased significantly (EM>P/EM><0.05), the ratio of C and E increased significantly.Conclusion The hepatic artery has suffered extensive morphological remodeling while portal hypertension, and in return affected the h