DNA甲基转移酶1和组蛋白去乙酰化酶1在子宫内膜异位症在位内膜的表达

目的 探讨DNA甲基转移酶1（DNMT1）和组蛋白去乙酰化酶1（HDAC1）在子宫内膜异位症患者子宫内膜中的表达及其两者在子宫内膜异位症发生、发展中的作用。方法 选取子宫内膜异位症患者在位内膜18例， 无子宫内膜异位症的对照子宫内膜20例，应用免疫组织化学法测定DNMT1的分布，应用免疫印迹法检测内膜组织中DNMT1和HDAC1蛋白的表达。结果 免疫组织化学提示，DNMT1主要分布于子宫内膜腺上皮细胞和间质细胞的胞核；子宫内膜异位症在位内膜中DNMT1的表达强度显著高于对照组子宫内膜（P<0.01）。免疫印迹发现，子宫内膜异位症在位内膜DNMT1和HDAC1蛋白的表达高于对照组子宫内膜, 差异有统计学意义（P<0.05）；且两者的表达呈正相关 （P<0.05）。结论 DNMT1和HDAC1在EMs患者的高表达可能在子宫内膜异位症的发生、发展中起着重要作用。

Expression of DNA methyltransferase 1 and histone deacetylase 1 in endometrium of women with endometriosis

Objective To investigate the alteration in the expression of DNA methyltransferase 1(DNMT1) and histone deacetylase 1(HDAC1) in endometrium from women with endometriosis and to explore the effects of both DNMT1 and HDAC1 on the endometriosis. Methods DNMT1 was localized with immunohistochemistry，DNMT1 and HDAC1 was quantified with Western blotting in the endometrium of 38 premenopausal women (18 with endometriosis and 20 without endometriosis). Results DNMT1 was predominantly located in the nuclei of glandular epithelial cells and stromal cells. The intensities of the DNMT1 immunohistochemical staining were significantly higher in the women endometrium with endometriosis than those without endometriosis(EM>P/EM><0.01）. DNMT1 and HDAC1 protein levels in the endometrium of women with endometriosis were significantly higher than those without endometriosis throughout the menstrual cycle（EM>P/EM><0.05）. There was a positive correlation between endometrial DNMT1 and HDAC1 levels in the endometriosis（EM>P/EM><0.05）.Conclusion High expression of DNMT1 and HDAC1 in the endometrium sug