尼古丁对大鼠血管平滑肌细胞增殖和凋亡的影响

目的 探讨尼古丁对大鼠胸主动脉血管平滑肌细胞(VSMCs)增殖和凋亡的影响.方法用终浓度为100 μmol/L的尼古丁作用于体外培养的大鼠VSMCs 24h,不加尼古丁的细胞作为对照组,应用荧光显微镜和扫描电子显微镜观察各组细胞凋亡的形态,BrdU-ELISA和流式细胞术检测各组细胞增殖和凋亡率,进而确定尼古丁对VSM...

Effect of nicotine on the proliferation and apoptosis of vascular smooth muscle cells in rats

Objective To investigate the effect of nicotine on the proliferation and apoptosis of rats’ aortic vascular smooth muscle cells (VSMCs) in order to demonstrate the mechanism of the smoking-induced the progress and mechanism of the atherosclerosis. Methods VSMCs isolated from rats were subjected to 100 μmol/L nicotine for 24 hours，and the cells without nicotine were used as control. The morphology of apoptotic cells was observed by the fluorescence microscope and scanning electron microscopy. The rate of cell apoptosis and proliferation was analyzed by flow cytometry (FCM). And also the proliferation of vascular smooth muscle cells was detected by BrdU(bromodeoxyuridine)-ELISA kit. Results Compared with the control group (14.46% ± 4.47%), the percent of S-phase cells in nicotine group reduced (6.77% ± 3.41%). The rate of the apoptosis VSMCs in control group was 11.47% ± 2.56%, which was lower than that in 100 μmol/L nicotine group (24.65%±4.67%, P<0.05). The DNA content detected by BrdU-ELISA in the nicotine group was remarkably lower than that in control group (P<0.05). Moreover, there were more cell apoptosis bodies and nucleorhexis in nicotine group than that in control group detected by cytochemical staining method and scanning electron microscope.Conclusion It is concluded that the steady dose