2型糖尿病大鼠动脉粥样硬化模型不同制备方法探讨

目的比较和探索制备2型糖尿病动脉粥样硬化大鼠模型的不同方法。方法健康雄性Wistar大鼠5只作为对照组,雄性自发性糖尿病模型GK大鼠随机分为高甘油三酯组(12周)、高胆固醇12周组和高胆固醇24周组,每组5只。组织病理学观察各组动脉粥样硬化形成情况。结果四组大鼠血管各层结构基本正常,未见典型动脉粥样硬化病变。免疫组织化学染色显示,四组主动脉壁均无巨噬细胞浸润,中膜及内膜层平滑肌细胞排列规则。结论对于2型糖尿病大鼠动脉粥样硬化模型的制备,这三种方法在Wistar大鼠均不易复制成功。

Evaluation of the Methods for Establishing Type 2 Diabetic Atherosclerotic Rat Models

Aim To compare and investigate the different methods for establishing type 2 diabetic atherosclerosis rat models. Methods 5 Wistar rats were chosen control group and 15 GK rats were randomly divided into three groups: hypertriglyceride diet (12 weeks) group, hypercholesterol diet with vitamin D (12 weeks) group and hyperchlesterol diet with vitamin D (24 weeks) group. Histopathology was used to observe the atherosclerotic lesions. Results There were no obvious morphologic changes in these four groups by light microscope. The macrophage-related antigen expressions in the aorta were not observed in all the four groups. Absence in abnormal expression of SMC-related antigens was shown in all of them. Conclusion All of the three methods were not successful in establishing diabetic atherosclerotic rat models in Wistar rats.