| 电子自旋共振法测定自由基两种标本处理方法的比较 |
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| 引用本文： | 姜招峰,金建国,周翔. 电子自旋共振法测定自由基两种标本处理方法的比较[J]. 吉林大学学报(医学版), 1989, 0(6) |
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| 作者姓名： | 姜招峰 金建国 周翔 |
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| 作者单位： | 白求恩医科大学生物化学教研室(姜招峰)，白求恩医科大学口腔医学系(金建国)，白求恩医科大学生物化学教研室(周翔) |
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| 摘 要： | 本文用电子自旋共振法(ESR)在直接冷冻和冰冻干燥两种不同条件下,测定了大鼠正常组织肝,肾、脑、血液中自由基含量。分析比较了两种不同条件下的测定结果,它们相应的g因子、线宽、强度等、均不相同。本文说明:直接冷冻法结果较真实可信;冰冻干燥法伪信号较强。
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| 关 键 词： | 电子自旋共振 直接冷冻 冰冻干燥 |
Comparison between two disposal methods .for biological sample in measurement of free radicals in various tissues with electron spin resonance |
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| Jiang Zhaofeng,Jin Jianguo and Zhou Xiang. Comparison between two disposal methods .for biological sample in measurement of free radicals in various tissues with electron spin resonance[J]. Journal of Jilin University: Med Ed, 1989, 0(6) |
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| Authors: | Jiang Zhaofeng Jin Jianguo Zhou Xiang |
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| Abstract: | Free radical levels in tissues of the normal rats, including the kidney, brain and blood were measured with electron spin resonance (ESR) after the biological samples were disposed with direct freezing and lyophilization methods respectively.The results of these two different conditions were analyzed and compared. Differences in g-value, linear width and signal intensity among these tissues were noticed. The results showed that the signal was real and reliable when direct freezing disposal method was used, and the pseudo-signal was more intense when lyophilization disposal method was used. |
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| Keywords: | Electron spin resonance Direct freezing Lyophilization |
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