北京地区腹泻儿童轮状病毒感染近况及3种检测试剂盒的比较

目的:了解北京地区腹泻患儿轮状病毒的感染情况,并比较3种试剂盒对轮状病毒的检出率。方法:对2004～2007年北京儿童医院门诊腹泻患儿43960份粪便标本进行轮状病毒抗原检测,分析发病季节、患儿年龄、性别与病毒检出率的关系;采用3种不同试剂盒(万泰、Coris和Asia-lion)对83份腹泻患儿的粪便标本同时进行轮状病毒抗原检测,并进行对比分析。结果:①轮状病毒检出率以秋冬季最高,其次为春季,夏季最低。同2005年和2006年相比,2007年检出率呈下降趋势。②662名不同年龄段患儿的检出率有显著性差异,其中以6~24月患儿检出率最高。③以两种试剂盒检测结果一致为标准,万泰、Coris和Asia-lion试剂盒检测轮状病毒的敏感性分别为93.3%(14/15)、100.0%(15/15)和86.7%(13/15);特异性分别为92.6%(63/68)、98.5%(67/68)和92.6%(63/68)。结论:北京地区儿童轮状病毒腹泻的发生率近3年呈下降趋势,高峰期仍在秋冬季节,但春季也有较高的发生率。2岁以下儿童轮状病毒感染率高。3种试剂盒均能对轮状病毒进行快速检测,准确性均较高。

Study on Rotavirus infection in diarrhea children of Beijing area and comparison of 3 test kits

Objective:To study the infection rate of Rotavirus in diarrhea children of Beijing area and to compare the detection ability of 3 different test kits.Methods:Rotavirus antigen was tested for 43 960 fecal samples of diarrhea children from the outpatient clinic of Beijing Children's Hospital between 2004 and 2007. The influence of season, age and sex on the detection rate of Rotavirus was analyzed. For 83 samples, Rotavirus antigen was tested with 3 different test kits (Wantai, Coris and Asia-lion)simultaneously and the results were compared.Results:①The detection rate of Rotavirus was the highest in autumn and winter, still high in spring and the lowest in summer. Compared with the year of 2005 and 2006, the infection rate decreased in 2007.②Significant difference of viral detection rate existed between different ages of 662 children and the highest in children aged 6-24 months.③Positive or negative of rotavirus infection was determined when the results of 2 test kits were in agreement. With this method as standard of reference, the sensitivity of Wanti, Coris and Asia-lion test kit was 93.3% (14/15), 100% (15/15) and 86.7% (13/15) and the specificity was 92.6% (63/68), 98.5%(67/68) and 92.6%(63/68) respectively.Conclusion:The infection rate of Rotavirus in diarrhea children of Beijing area is decreasing in recent 3 years. The peak period is still in autumn and winter, but is also high in spring. The infection rate is high in children under 2 years old.All the 3 test kits could detect Rotavirus antigen rapidly and the diagnostic accuracy is acceptable.