皮肤鳞状细胞癌中Basigin/CD147表达与肿瘤进展的相关性

目的 探讨Basigin/CD147在皮肤鳞状细胞癌(鳞癌)进展中的作用及其机制.方法 运用免疫组化方法检测36例浸润和复发/转移情况不同的原发皮肤鳞状细胞癌标本中Basigin和基质金属蛋白酶(MMPs)的表达.运用免疫印迹和免疫荧光法观察Basigin在培养角质形成细胞和鳞状细胞癌细胞中的表达和定位.结果 有浸润和伴复发/转移的原发鳞癌肿瘤细胞中Basigin、MMP-1、MMP-2和MT1-MMP的表达显着增高.肿瘤细胞周围成纤维细胞中MMP-1、MMP-2和MT1-MMP的表达亦与原发鳞癌的复发/转移密切相关.肿瘤细胞膜上Basigin的表达与成纤维细胞中MMP-1、MMP-2、MMP-3和MT1-MMP的表达间显着相关.增高表达的Basigin主要分布于鳞癌细胞膜的微绒毛上.结论 鳞癌细胞膜上增高表达的Basigin/CD147可能与周围成纤维细胞作用,诱导其MMPs产生,并通过这一机制在鳞癌进展中发挥重要作用.

The Correlation Between Basigin/CD147 Expression in Cutaneous Squamous Cell Carcinoma and Tumor Progression

Objective To investigate a possible role of basigin /CD147and a mechanism how basig in /CD147functions in the progression o f cutaneous squamous cell carcinoma (SCC).Methods Expression of basigin and matrix metalloproteinases(MMPs )was examined immunohistochemicall y in 36specimens taken fromthe patie nts with SCC.Expression was compared between 7SCC in situ and 29invasive SCC as well as 19SCC without recur-rence /metastasis and 17with recurrence /metastasis.Immunoblot analy sis and immunofluorescence microsc opy were used to observe the expression and lo calization of basigin in SCC cells in vitro.Results Expression of basigin in primary SCC increased during the pro gression of tumor invasion.Primary SCC with recurrence /metastasis exh ibited significantly increased basigin expression compared to primary SCC wit hout recurrence /metastasis.There were sig-nificant differences between SCCin situ and invasive SCCin the expression of MMP -1,MMP -2and MT1-MMP in tumor cells and between SCC with and without recurrence /metastasis in th e expression of MMP -1,MMP -2and MT1-MMP in both tumor cells and fibro blasts.Significant correlation wa s found between basigin expression i n tumor cells and MMP -1,MMP -2,MMP -3and MT1-MMP expression in surrounding fibr oblasts.Immunoblot analysis revealed increased basigin expression in culture SCCcells.Basigin was found to localize on microvilli -lik e structures on cell membranes of tumor cells by im munofluorescence microscopy.Conclusions These results suggest that basigin /CD147expressed in SCCcells might interact with surrounding fibroblasts to stimulate their MMPs ex pression and play an important role in the prog ression of SCC through this interaction.