优化柱前衍生-HPLC法测定血清中丙戊酸的血药浓度

目的：优化柱前衍生-高效液相色谱法监测血清中丙戊酸浓度的方法。方法：以乙腈为蛋白沉淀剂，以弘溴苯乙酮为衍生化试剂进行测定。色谱柱为ANAX丙戊酸专用分析柱，流动相为乙腈-水（70：30），柱温为40℃，流速为0．7mL·min-1，检测波长为266nm。结果：丙戊酸血药浓度在4．88-139．46μg·mL-1范围内线性关系良好（r=0．9996），平均回收率为100．01％，日内、日间RSD均〈3％。结论：本法可简便、快速、稳定地测定血清中丙戊酸的浓度，能满足临床监测的要求。

Determination of Valproic Acid Concentration in Serum by Optimized HPLC after Pre-column Derivatization

OBJECTIVE： To optimize HPLC after pre-column derivatization to monitor serum concentrations of valproic acid. METHODS： The separation was performed on ANAX column with acetonitrile-water （70：30） as mobile phase at the flow rate of 0.7 mL·min-1 with detection wavelength of 266 nm using acetonitrile as protein precipitants and a-bromoacetophenone as derivating agent. RESULTS： The linear range of valproic acid was 4.88- 139.46 μg·mL-1（r= 0.999 6） with mean recovery of 100.01%. The RSDs of inter-day and intra-day were less than 3 %. CONCLUSION： This method is simple, rapid and stable for the determination of valproic acid concentration in serum, and it meets the demand of clinical monitoring.