一种骨种子细胞体外应力培养系统的建立

目的建立体外应力培养系统，观察应力刺激对骨种子细胞成骨分化的影响。方法选择具有明确成骨分化潜能的间充质干细胞(MSCs)作为种子细胞，以脱细胞骨基质为支架材料，以流体切应力作为对种子细胞的体外应力刺激。建立一种骨种子细胞体外三维应力培养系统——流动腔灌流体系，并利用该系统对MSCs的碱性磷酸酶(ALP)活性和骨钙素产物的影响进行评价。结果该系统可以明显促进ALP活性和骨钙素产物的表达，而细胞计数无明显改变。结论本系统为骨组织工程研究提供了一种有效的体外培养模型。

Establish a Fluid Flow Shear Stress Model for Culturing Bone Engineering Seeded Cells in Vitro

Objective To study the effect of fluid flow shear stress on seeded cells for bone tissue engineering. Methods In this study we established a flow chamber perfusion system. The mesenchymal stem cells(MSCs) were implanted into the acellular bone exteracellular matrix, then cultured in the perfusion system under 1.0dyn/cm2 flow shear stress for 7days. Results Alkaline phosphatase(ALP) activity and osteocalcin content, which were two crucial osteoblast genes marker were increased. Cell numbers had no different with the static group. Conclusion It demonstrated that this system is useful for osteoblastic differentiation of bone seeded cells, and this 3D cultured model utilizing fluid flow may be helpful in the culture process of engineering bone.