原核表达戊型肝炎病毒基因重组结构蛋白免疫保护恒河猴的初步实验研究

目的 观察基因重组戊型肝炎病毒（HEV）结构蛋白对恒河猴戊型肝炎野病毒攻击的保护作用。方法 用HEV重组结构蛋白免疫恒河猴，猴血清中抗-HEV升高后，与空白对照组一同用HEV野病毒攻击，采血观察野病毒攻击前后ALT和HEV抗体等的动态变化。结果 野病毒攻击后第3周，空白对照组5只猴ALT均出现明显异常，而免疫接种组5只猴ALT均正常，未观察到明显的肝脏炎症表现，结论 HEV重组结构蛋白免疫恒河猴之后，可以有效地保护HEV野病毒的攻击，该HEV重组结构蛋白可作为戊型肝炎基因工程疫苗的候选蛋白。

Preliminary evidence that a hepatitis E virus(HEV)ORF2 recombinant protein protects cynomolgus macaques against challenge with wild-type HEV

Objective To observe the protective effict of hepatitis E virus(HEV)ORF 2 recombinant protein expressed in prokaryote cell cynomolgus macaques(cynos)against challenging with wild-type HEV.Methods Cynos were immunized with HEV ORF 2 recombinant protein and then challenged with wild-type HEV,the unimmunized cynos were used as control.Blood samples were collected and tested to see if there were dynamic changes of ALT and antibody to HEV before and after challenge with wild-type HEV.Results All the five unimmunized cynos re-presented hepatitis 3 weeks after challenging with wild-type HEV.However,all the five immunized cynos showed no hepatitis and pathological changes.Conclusion Cynos can be efficiently protected by immunization with HEV ORF 2 recombinant protein against wild-type HEV.This protein can be a promising candidate for HEV vaccine.