非小细胞肺癌ERK、AKT蛋白磷酸化的病理意义

目的:探讨非小细胞肺癌ERK、AKT蛋白磷酸化的病理意义。方法:免疫组织化学SP法检测90例非小细胞肺癌的p-ERK和p-AKT表达状况，分析二者表达与通路上游EGFR、KRAS、PI3K表达及患者部分临床病理参数间的相关关系。结果:非小细胞肺癌中，p-ERK表达阳性率为53.33%，其与患者的性别和吸烟史及肺腺癌的分化程度相关（P<0.05）。p-ERK表达与EGFR及KRAS表达均无显著相关关系（P>0.05）。p-AKT表达阳性率为56.67%，p-AKT表达与EGFR表达显著正相关（r=0.336，P<0.05），但与PI3K表达无显著相关关系（P>0.05）。p-ERK与p-AKT表达显著正相关（r=0.292，P<0.05）。结论:非小细胞肺癌中可能存在ERK的自身激活，检测ERK与AKT蛋白的磷酸化状态可能对筛选EGFR-TKI治疗获益人群有所帮助。

The pathological significance of ERK and AKT phosphorylation in non -small cell lung cancer

Objective:To investigate the pathological significance of ERK and AKT phosphorylation in non -small cell lung cancer (NSCLC).Methods:Immunohistochemistry was used to detect the expression of p -ERK,p -AKT and their upstream proteins in 90 NSCLC samples,and the SPSS 13.0 statistic software was used to analyze the corre-lations of p -ERK and p -AKT protein expressions to patients'clinical pathological agents and their upstream protein expressions.Results:The positive rate of p -ERK protein expression in NSCLC was 53.33%,associating with gen-der,smoking status of patients and differentiation of the adenocarcinoma (P <0.05).While no significant relation-ships were found between p -ERK protein expression and EGFR,KRAS protein expressions (P >0.05).The positive rate of p -AKT protein expression in NSCLC was 56.67%.p -AKT protein expression was correlated with EGFR protein expression (r =0.336,P <0.05),but PI3K protein expression (P >0.05).p -ERK protein expression was correlated with p -AKT protein expression (r =0.292,P <0.05).Conclusion:The ERK protein might be activated spontaneously in NSCLC,and the detection of p -ERK and p -AKT protein expressions would be helpful to select pa-tients benefiting from EGFR -TKI therapy.