二甲双胍对人滤泡状甲状腺癌细胞生长和能量代谢的影响

目的:观察不同浓度二甲双胍对人滤泡状甲状腺癌(follicular thyroid cancer，FTC）细胞增殖、凋亡及能量代谢的影响。方法:体外培养人FTC细胞(FTC-133)，分为对照组和不同浓度二甲双胍处理组（1、3和10mmol/L），分别培养24h、48h和72h。四甲基偶氮唑蓝（MTT）法检测细胞增殖，Annexin V-FITC/PI流式细胞术检测细胞凋亡，Seahorse能量代谢分析仪检测细胞糖酵解及线粒体呼吸的变化。结果:与对照组相比，二甲双胍抑制FTC-133增殖，随浓度及处理时间的增加，抑制效应加大，呈剂量-时间依赖性（P＜0.05）。二甲双胍诱导FTC-133凋亡，随浓度及处理时间的增加，其中晚期凋亡率明显增加，呈剂量-时间依赖性（P＜0.05）。二甲双胍可影响FTC-133糖代谢，与对照组相比，随二甲双胍浓度增加，糖酵解和线粒体呼吸可明显抑制，呈剂量-时间依赖性（P＜0.05）。结论:二甲双胍抑制FTC-133增殖并诱导凋亡，其机制可能是影响癌细胞能量代谢进而抑制其能量供应。

The effect of metformin in growth and energy metabolism of follicular thyroid cancer cell

Objective:To investigate the effects of different concentration metformin on proliferation,apoptosis and energy metabolism of follicular thyroid cancer(FTC). Methods:After treating with metformin(0,1,3,10mmol/ L), the viability of FTC cell line(FTC - 133)was analyzed by 3 -(4,5 - dimethyl - thiazol - 2 - yl)- 2,5 - dipheny-ltetrazoliumbromide(MTT). Cell cycle analysis was performed by AnnexinV - FITC/ PI flow cytometry,the energy metabolism of FTC - 133 was tested by Seahorse. Results:Compared with control group,MTT assays showed that the proliferation of FTC - 133 was inhibited in a dose - time dependent manner after treating with metformin(P < 0. 05). Moreover,metformin induced the late apoptosis of FTC - 133(P < 0. 05). Metformin changed the pattern of cell ener-gy metabolism,manifested with a significant decrease in glycolysis and mitochondrial respiration of FTC - 133 in a dose - time dependent manner(P < 0. 05). Conclusion:Metformin provided an anti - tumor effect in FTC - 133 which may be mediated by decreasing the energy supply of cancer cells.