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| Canstatin RNA转染对兔血管平滑肌细胞体外增殖的抑制作用 | |
| 引用本文： | 李浪,冯建章,郑文岭,冼江,张宏斌,杨太成,唐其东,邓春玉.Canstatin RNA转染对兔血管平滑肌细胞体外增殖的抑制作用[J].中国病理生理杂志,2001,17(8):746-748. |
| 作者姓名： | 李浪 冯建章 郑文岭 冼江 张宏斌 杨太成 唐其东 邓春玉 |
| 作者单位： | 1. 广东省心血管病研究所,广东省人民医院霍英东心脏中心,广东广州510080; 2. 广州军区总医院分子肿瘤研究所,广东广州510010; 3. 广西医科大学心血管病研究所心内科,广西南宁530027 |
| 基金项目： | 广东省医学科学研究基金资助项目(No.A2000054) |
| 摘 要： | 目的:探讨canstatin对体外培养的兔血管平滑肌细胞(VSMC)增殖的影响。方法:将阳离子脂质体介导的canstatinRNA转染VSMC,应用细胞计数,3H]-TdR掺入率测定观察canstatin基因对VSMC增殖的作用。结果:阳离子脂质体介导的canstatinRNA可有效地转染VSMC并能显著地抑制VSMC的增殖及其DNA的合成率。结论:Canstatin基因可抑制体外培养的VSMC的增殖. |
| 关 键 词： | 基因转移 肌 平滑 血管 RNA |
| 文章编号： | 1000-4718(2001)08-0746-03 |
| 收稿时间： | 2001-01-15 |
| 修稿时间： | 2001年1月15日 |
Inhibitory effect of canstatin RNA transfection on the growth of cultured rabbit vascular smooth muscle cells |
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| LI Lang ,FENG Jian-zhang ,ZHENG Wen-ling ,XIAN Jiang ,ZHANG Hong-bin ,YANG Tai-cheng ,TANG Qi-dong ,DENG Chun-yu.Inhibitory effect of canstatin RNA transfection on the growth of cultured rabbit vascular smooth muscle cells[J].Chinese Journal of Pathophysiology,2001,17(8):746-748. | |
| Authors: | LI Lang FENG Jian-zhang ZHENG Wen-ling XIAN Jiang ZHANG Hong-bin YANG Tai-cheng TANG Qi-dong DENG Chun-yu |
| Institution: | 1. Department of Cardiology,Guangdong Cardiovascular Institute,Guangdong People's Hospital,Guangzhou 510080,China; 2. Institute of Molecular Oncology,Liu Hua Qiao Hospital,Guangzhou 510010,China; 3. Department of Cardiology,Cardiovascular Institute,Guangxi Medical University,Nanning 530027,China |
| Abstract: | AIM: To investigate the effect of canstatin on cultured rabbit vascular smooth muscle cells(VSMC). METHODS: By means of cationic liposome mediated method, canstatin RNA was transferred into cultured VSMC. The proliferation quantity of VSMC were determined by the cell counting method and thymidine(-TdR) incorporation. RESULTS: Canstatin RNA could be effectively transferred into cultured primary rabbit aortic smooth muscle cells by the cationic liposome-Dosper and could markedly inhibit VSMC proliferation. CONCLUSION: Transfection of canstatin RNA could inhibit the growth of VSMC in vitro. |
| Keywords: | Gene transfer Muscle smooth vascular RNA |
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