三取代型钛钨硅酸盐体内抑瘤效应的免疫机制探讨

目的:探讨三取代型钛钨硅酸盐(WT)体内抑瘤效应的免疫机制。方法:建立荷H22肝癌小鼠模型,WT连续灌胃10d,取出肿瘤称重测定抑瘤率。用MTT比色法测定荷H22肝癌小鼠淋巴细胞转化的活性及NK细胞的杀伤活性。通过形态学观察和流式细胞仪检测WT诱导BEL-7402细胞的凋亡。结果:WT可显著抑制荷瘤小鼠肿瘤生长(P<0.05),提高荷瘤小鼠淋巴细胞转化的活性及NK细胞的杀伤活性(P<0.05),并诱导肿瘤细胞发生凋亡。结论:WT的抑瘤作用与机体免疫功能的增强有关。

Investigation of immunological mechanism of the in vivo antitumor effect of K8 (C6H11N3O2)2[SiW9Ti3O40] (WT)

AIM: To investigate the in vivo antitumor effect of K_8(C_6H_11N_3O_2)_2[SiW_9Ti_3O_(40)] (WT) and the immunological mechanism. METHODS: WT was administrated intragastrically to mice bearing H22 hepatocarcinoma for 10 days and then the tumors were isolated and weighed. MTT colorimetry was used to detect lymphocyte transformation function and cytotoxicity of NK cells. The morphology of apoptotic tumor cells was observed under phase contrast microscope and light microscope after HE staining. The apoptosis rate of BEL-7402 cells treated with WT was detected by flow cytometry. RESULTS: Compared to the control group, WT obviously inhibited the growth of H22 tumor in tumor bearing mice (P<0.05). It enhanced significantly lymphocyte transformation function and cytotoxicity of NK cells (P<0.05). Apoptotic tumor cells were observed under microscope and detected by flow cytometry. CONCLUSION: WT inhibits significantly the growth of tumor by up-regulating the activity of immunocytes and inducing apoptosis of tumor cells.