| 油橄榄叶中活性成分的提取工艺考察 |
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| 引用本文： | 王和宇,李春娜,秦玲玲,张晶,徐暾海,刘铜华.油橄榄叶中活性成分的提取工艺考察[J].甘肃中医,2017,30(3). |
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| 作者姓名： | 王和宇 李春娜 秦玲玲 张晶 徐暾海 刘铜华 |
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| 作者单位： | 1. 北京中医药大学中医养生学教育部重点实验室,北京 100029;吉林农业大学中药材学院;2. 北京中医药大学中医养生学教育部重点实验室,北京 100029;北京中医药大学中药学院;3. 北京中医药大学中医养生学教育部重点实验室,北京,100029;4. 吉林农业大学中药材学院 |
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| 基金项目： | 国家国际科技合作专项项目，北京中医药大学创新团队项目 |
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| 摘 要： | 目的:考察油橄榄(Olea europaea Linn)叶中活性成分的最佳提取工艺。方法:采用HPLC法测定油橄榄叶中木犀草苷、橄榄苦苷,并使用超声波提取法进行提取,考察料液比,溶剂分数,提取时间,超声功率对含量的影响。在单因素基础上,结合正交试验考察溶剂分数、料液比、提取时间对橄榄苦苷含量的影响,确定最佳提取工艺。结果:油橄榄叶中活性成分的最佳提取工艺为80%甲醇溶液30 mL,超声400W提取30分钟,提取次数1次。结论:采用该方法提取油橄榄叶中活性成分简单易行。
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| 关 键 词： | 油橄榄叶 HPLC 木犀草苷 橄榄苦苷 超声波提取法 正交试验 提取工艺考察 |
Extraction Technology for Active Components in Olive Leaves |
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| WANG Heyu,LI Chunna,QIN Lingling,ZHANG Jing,XU Dunhai,LIU Tonghua.Extraction Technology for Active Components in Olive Leaves[J].Gansu Journal of Traditional Chinese Medicine,2017,30(3). |
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| Authors: | WANG Heyu LI Chunna QIN Lingling ZHANG Jing XU Dunhai LIU Tonghua |
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| Abstract: | Objective:To study the best extraction technology for active components in olive leaves. Methods:Galuteolin, oleuropein in olive leaves were determined by HPLC method, and extracted by ultrasonic method. Ef-fects of the solid-liquid ratio, solvent fraction, extraction time and ultrasonic power on contents were inspected. Based on single factor tests and combined with orthogonal test, effects of the solvent fraction, solid-liquid ratio and extraction time on the content of oleuropein were inspected to determine the best extraction technology. Results:The best extraction technology for active components in olive leaves was extracting for 30min with 30mL 80%methanol solution under the condition of 400W ultrasound, and the extraction time was once. Conclusion:This method is sim-ple and practicable for extracting the active components in olive leaves. |
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| Keywords: | olive leaves HPLC galuteolin oleuropein ultrasonic extracting method orthogonal test study of the extraction technology |
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