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马钱子总生物碱含量测定方法的建立与比较
引用本文:王咏,陈军. 马钱子总生物碱含量测定方法的建立与比较[J]. 西部中医药, 2017, 30(7). DOI: 10.3969/j.issn.1004-6852.2017.07.008
作者姓名:王咏  陈军
作者单位:1. 盐城卫生职业技术学院,山东盐城,224005;2. 南京中医药大学药学院
基金项目:广东省自然科学基金项目
摘    要:目的:建立简便易行的马钱子总生物碱含量测定方法并评价马钱子总生物碱提取工艺。方法:建立应用紫外分光光度法测定马钱子总生物碱含量的方法,并与常规的比色法以及HPLC法同时测定不同提取工艺下马钱子总生物碱样品的含量。结果:3种方法的测定结果平行,相互可以参照。紫外分光光度法可以用于马钱子总生物碱的含量测定。

关 键 词:马钱子  总生物碱  含量测定  紫外分光光度法

The Comparison and Establishment of Methods for Determining the Contents of Total Alkaloid Extracted from MaQianZi
WANG Yong,CHEN Jun. The Comparison and Establishment of Methods for Determining the Contents of Total Alkaloid Extracted from MaQianZi[J]. Western Journal of Traditional Chinese Medicine, 2017, 30(7). DOI: 10.3969/j.issn.1004-6852.2017.07.008
Authors:WANG Yong  CHEN Jun
Abstract:Objective:To assess the extraction technology of total alkaloids from MaQianZi (nuces vomicae) by establishing simple and convenient methods to determine the contents of total alkaloids from MaQianZi.Methods:The method of determining the contents of total alkaloids from MaQianZi was established by using ultraviolet spectrophotometry,and the contents of total alkaloids from MaQianZi under different extraction technology were determined by conventional colorimetry and HPLC method simultaneously.Results:The determination results of three methods were paralellel,which could be cousulted by each other.Conclusion:Ultraviolet spectrophotometry could be used for content determination of total alkaloids from MaQianZi.
Keywords:MaQianZi  total alkaloids  content determination  ultraviolet spectrophotometry
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