高效液相色谱法测定薛草茎中木樨草素的含量

目的:建立释草茎中木樨草素的定量检测HPLC方法,考察律草茎的木樨草素含量.方法:高效液相色谱法中固定相为Kromasil C18分析柱(200 × 4.6 mm,5m),流动相为甲醇:0.2％磷酸水溶液(55:45),检测波长为350 nm,流速为1 mL/min,柱温为30℃.结果:木樨草素在0.03～0.46 μg范围内浓度与峰面积呈良好线性关系.线性方程为Y=2 607 187X-19 694,r=0.999 4,平均回收率为98.8％,RSD=1.77％ (n=5).结论:所建立的方法简便,准确,专属性强,灵敏度高,重现性好,可用于葎草中木樨草素的含量测定.

The Contents of Luteolin in the Stems of Humulus Japonicus Determined by HPLC

Objective:To establish the quantitative detection methods for determining the contents of luteolin in the stems of humulus japonicus by HPLC,and investigate the contents of luteolin in the stems of humulus japonicus.Methods:Stationary phase of HPLC was Kromasil C18 analytical column (200×4.6 mm,5 m),mobile phase was:methanol:0.2％ aqueous phosphate solution (55:45),detection wavelength was 350 nm,flow rate was 1 mL/min,column temperature:30℃.Results:The concentrations and peak area of luteolin were in good linear relationship in the range from 0.03 to 0.46 μg.Linear equation was:Y=2 607 187X-19 694,r=0.999 4,average recovery rate was 98.8％,RSD=1.77％(n=5).Conclusion:The method,simple,accurate,with high specificity,high sensibility and good repeatability,could be used for content determination ofluteolin in the stems ofhumulus japonicus.