一步法PCR联合荧光原位杂交检测曲霉菌属感染标本

目的 联合新颖的一步法PCR检测法和荧光原位杂交(FISH)检测法,检测病理诊断曲霉菌属感染标本.方法 收集病理诊断曲霉菌属感染标本33份作为试验组；随机收集非曲霉菌属感染标本12份作为阴性对照组；采用标准黑曲霉菌株作为阳性对照组；建立新颖的一步法PCR检测技术,联合FISH检测法对试验组及对照组标本进行检测.结果 一步法PCR可以快速、特异地检测量仅0.01 g的黑曲霉菌丝；试验组中,有16份标本两种检测均为阳性,12份检测均为阴性；所有阴性对照组标本两种检测均为阴性.结论 一步法PCR检测曲霉菌属具有很高的检测灵敏性和特异性,其联合FISH检测,可作为一种有潜力的曲霉菌属快速检测方法.

One-step PCR combined with fluorescence in situ hybridization detection of specimens infected with Aspergillus

OBJECTIVE To detect the specimens infected with Aspergillus by novel one-step PCR combined with fluorescence in situ hybridization(FISH).METHODS A total of 33 specimens with pathologically suspected Aspergillus infections were collected as experimental group;12 specimens without Aspergillus infection were randomly collected as the negative control group;a standard Aspergillus niger was collected as a positive control group;a novel one-step PCR method was established to combine with FISH to detect the specimens in the experimental group and the control group.RESULTS The one-step PCR could rapidly and specifically detect the Aspergillus niger with only 0.01g mycelia;totally 16 specimens in experimental groups were detected positive with both one step PCR and FISH,12 specimens detected negative;the specimens in the negative control group were detected negative.CONCLUSION One-step PCR is with high sensitivity and specificity in the detection of Aspergillus,and its combination with FISH can serve as an potential rapid detection of Aspergillus.