铜绿假单胞菌抗菌制剂外排泵基因与可移动遗传元件研究

目的 研究临床分离铜绿假单胞菌(PAE)抗菌制剂外排泵基因与可移动遗传元件存在状况.方法 分离自贵州省贵阳地区(A组)和江苏省苏南地区(B组)PAE各20株,用纸片扩散法测定19种抗菌药物的敏感性,采用PCR法检测抗菌制剂外排泵基因(smr、smr-2、qacE△1),质粒遗传标记(traF、trbC),插入序列遗传标记(IS1133、ISEcp1),转座子遗传标记(merA、tnpU、tnp513),整合子遗传标记(qacE△1又为Ⅰ类整合子遗传标记)等可移动遗传元件；对阳性基因进行测序,测序结果进行Biast分析.结果 两组PAE呈β-内酰胺类、氨基糖苷类、喹诺酮类多药耐药；A组检出qacE△1、merA等两种基因,阳性率分别为10.0％、5.0％；B组检出qacE△1、merA、tnpU、tnp513等4种基因,阳性率分别为100.0％、85.0％、5.0％、55.0％.结论 两组为多药耐药的表型,与携带抗菌制剂外排泵基因和可移动遗传元件相关.

Investigation of drug efflux genes and mobile genetic elements in Pseudomonas aeruginosa

OBJECTIVE To investigate the distribution of drug efflux genes and mobile genetic elements in Pseudomonas aeruginosa(PAE)isolated from clinic.METHODS A total of 40 strains of P.aeruginosa were collected,20 from Guiyang area in Guizhou province assigned as group A and another 20 from Sunan area in Jiangsu province as group B.The antimicrobial susceptibility test(AST) was performed for 19 antibiotics with K-B method,and the drug efflux pump genes(smr,smr-2,qacE△1) and mobile genetic elements including genetic markers of the plasmid(traF,trbC),insert sequence(IS1133,ISEcp1),transposon(merA,tnpU,tnp513) and integron(qacE△,the first kind of integron genetic markers) were amplified by PCR,then the positive genes were sequenced and the sequencing results were analyzed by Blast.RESULTS PAEs in both two groups were multidrug-resistant to β-lactamases,aminoglycoside and fluoroquinolones;qacE△1 and merA were detected in group A with the positive rates of 10.0% and 5.0%,respectively;qacE△1,merA,tnpU and tnp513 genes were detected in group B with the positive rates of 100.0%,85.0%,5.0% and 55.0%,respectively.CONCLUSION The multidrug-resistant phenotype of PAE is associated with drug efflux genes and mobile genetic elements.