辽藁本药材RP-HPLC指纹图谱研究

目的建立辽藁本的高效液相色谱指纹图谱,研究不同产地辽藁本的质量,建立评价辽藁本质量优劣的指纹图谱分析方法。方法利用RP-HPLC方法,梯度洗脱,测定了16批辽藁本样品。色谱柱：Dikma C18柱（200mm×4.6mm,5μm）;流动相：A相为乙腈,B相为磷酸水溶液,在0-15min,B相从90%线形改变至80%,15-60min,B相从80%线形改变至15%;流速：0.8mL.min^-1;检测波长：274nm;柱温：25℃;进样量：10μL。结果建立了RP-HPLC指纹图谱共有模式。结论辽藁本药材的高效液相色谱指纹图谱重复性好,专属性强,可用于辽藁本药材的质量控制。

RP-HPLC fingerprint spectrum of Rhizoma et Radix Ligustici

OBJECTIVE To establish the chromatographic fingerprint analysis for the quality control of Rhizoma et Radix Ligustici.METHODS RP-HPLC method was applied to establish the chromatographic fingerprint.The reversed phase HPLC system consisting of a C18 column（200mm×4.6mm,5μm）and a mixture of acetonitrile（A）and 0.04% phosphoric acid （B）with gradient elution（0～15min,90%→80% B,15～60min,80%→15% B,volume fraction）as the mobile phase was adopted.The column temperature was set up at 25℃ and the flow-rate was 0.8 mL.min-1.The absorbance was monitored at 274nm.RESULTS RP-HPLC fingerprint have set up and owned the mode in common.,with better relevance.CONCLUSION The RP-HPLC fingerprint method is repeatable,feasible in analysis of Rhizoma et radix ligustici and can be used scitentifically to evaluate the quality of Rhizoma et radix ligustici.