蛇床子中的细胞毒活性香豆素

研究植物蛇床Cnidium monnieri干燥成熟果实中的香豆素类成分及其细胞毒活性,为进一步开发利用蛇床子提供依据。采用大孔树脂、硅胶和Sephadex LH-20、C₁₈柱色谱等方法分离纯化,并运用波谱方法对所分离的化合物进行结构鉴定。采用MTT法对所分离得到的化合物进行L1210癌细胞毒活性实验。从蛇床子中分离鉴定了11个香豆素类化合物,分别为蛇床子素(1)、佛手柑内酯(2)、花椒毒酚(3)、花椒毒素(4)、欧前胡素(5)、异虎耳草素(6)、欧前胡素酚(7)、补骨脂素(8)、5,7-二甲氧基香豆素(9)、水合羟基前胡内酯(10)、swietenocoumarin F(11)。化合物 7,9~11 为首次从蛇床属植物中分离得到。在样品溶液浓度为1×10^-5 mol·L^-1的条件下,化合物 1,5,10, 11 对小鼠白血病L1210细胞体外生长的抑制率分别是70.13%,63.10%,55.77%,75.08%,表明上述化合物对L1210细胞具有较明显的体外细胞毒活性。

Studies on coumarins from fruit of Cnidium monnieri and their cytotoxic activities

This study is to study is to investigate the coumarins from Fruit of Cnidium monnieri and their cytotoxic activities. The constituents were separated by column chromatography,and their structures were elucidated by spectroscopic data analyses. The isolated compounds were evaluated for their cytoxic activities by MTT method. Eleven compounds were isolated and identified as osthole(1), bergaptan(2), xanthotoxol(3), xanthotoxin(4), imperatorin(5), isopimpinellin(6), osthenol(7), psoralen(8), 5,7-dimethoxycoumarin(9), oxypeucedaninhydrate(10), and swietenocoumarin F(11). Compounds 7, 9-11 were isolated from the Cnidium genus for the first time.Compounds 1,5,10 and 11 showed significant cytotoxic activities against L1210 cell lines at a concentration of 1×10^-5 mol·L^-1 with inhibitory rates of were 70.13, 63.10, 55.77, and 75.08% respectively.