脂蛋白脂酶基因PvuⅡ位点多态性与高脂血症人群膳食干预易感性的关系

为了解脂蛋白脂酶 (LPL)基因PvuⅡ位点多态性与高脂血症人群膳食干预效果的关系 ,从北京市西城区 8个社区居民中筛出 43 6名高脂血症患者 ,分为干预组 (2 48人 )和对照组 (188人 ) ;对两组人群进行血脂谱水平检测、膳食调查、体格检查及LPL基因PvuⅡ位点多态性检测 (PCR RFLP方法 ) ,并对干预组进行为期 6个月的膳食干预。结果表明与对照组相比 ,干预组膳食总能量、脂肪供能比及胆固醇摄入量明显降低 ,血清TC、LDL C和HDL C水平明显下降 (P <0 0 5 ) ,且LPL PvuⅡ位点 (+ +)基因型携带者TC和LDL C水平降低幅度大于 (+ - )及 (- - )基因型 ;多元线性回归分析显示膳食干预易感的因素包括LPL(+)等位基因、基限TC、LDL C水平较高以及超重等。本研究可初步得出LPL基因PvuⅡ位点 (+)等位基因与高脂血症人群膳食干预易感性密切相关 ,但限于研究对象的代表性该结论还需要进一步研究支持

Relations of lipoprotein lipase gene polymorphism at PvuⅡ locus and dietary intervention predisposition in hyperlipidemia population

In order to investigate the relation of lipoprotein lipase (LPL) gene polymorphism at PvuⅡ locus and dietary intervention predisposition in hyperlipidemia population., 436 hyperlipidemia patients were screened selected from some communities in western urban districts in Beijing, and then separated into two groups, intervention (248) and control group(188). The serum lipids profile, dietary intakes data were collected and physical examination was conducted in all subjects. Also, the LPL PvuⅡ polymorphisms were analyzed by PCR RFLP. The results showed that the total energy intake, cholesterol level and percentage of energy from dietary fat, and the serum levels of TC, LDL C and HDL C were significantly decreased ( P <0 05) after 6 month dietary intervention in intervention group compared with the control group. The decrease range of TC and LDL C in carriers with LPL PvuⅡ (+/+) genotype was much more than others(+/- and -/- genotypes). The results from multiple linear regression analysis showed that the susceptible factors of dietary intervention included LPL PvuⅡ (+) allele, the high baseline levels of TC and LDL C and overweight. The conclusions could be primarily drawn that the variants of LPL-PvuⅡ locus were important determinants of variation in serum cholesterol response to dietary change in hyperlipidemia population.