消癥注射液对子宫内膜异位症异位内膜凋亡率的影响

[目的]探讨消癥注射液治疗子宫内膜异位症的机制.[方法]测量用药前后异位病灶的体积变化,光镜观察变化;采用流式细胞术检测不同组别异位内膜及在位内膜的凋亡率和细胞周期的变化.[结果]测量各组用药前后的体积变化有显著差异(P＜0.05),消癥注射液组光镜下表现为腺腔数目减少,形态改变;流式细胞术检测各组异位内膜均存在细胞凋亡,模型组凋亡率下降,而用药组凋亡率上升,细胞周期被阻滞于S期而导致凋亡出现.[结论]消癥注射液能通过均可以明显抑制异位病灶的生长,阻滞细胞周期于S期,导致异位内膜出现凋亡,而促进了异位病灶的缩小至消失,达到治疗目的.

Effect of XiaoZheng injection on apoptosis rate of ectopic endometrium in endometriosis

[Objective] To investigate the effect mechanism of Xiaozheng injection in treating endometriosis.[Methods] The size of ectopic endometrium of rats was measured before and after medication.The histologic changes were observed with light microscope.The apoptosis rate and the change of cell cycle of each group were detected by FCM.[Results] The change of size before and after medication was significantly different between each groups(P<0.05).Xiaozheng injection groups could decrease the number of glandular cavities under microscopy.Morphologic changes were also found.The FCM showed that the apoptosis of the ectopic endometrium was existed in each group.The cell cycle was blocked at S stage that resulted in apoptosis.[Conclusions] Xiaozheng injection may promote ectopic endometrium diminished to disappeared by blocking cell cycle at S stage.