Molecular survey of rodent-borne Trypanosoma in Niger with special emphasis on T. lewisi imported by invasive black rats |
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Authors: | Dobigny Gauthier Poirier Philippe Hima Karmadine Cabaret Odile Gauthier Philippe Tatard Caroline Costa Jean-Marc Bretagne Stéphane |
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Affiliation: | aInstitut de Recherche pour le Développement, Centre de Biologie pour la Gestion des Populations, Centre Régional Agrhymet, BP 11011, Niamey, Niger;bCentre de Biologie et de Gestion des Populations (UMR IRD-INRA-Cirad-Montpellier SupAgro), Campus de Baillarguet, CS30016, 34988 Montferrier-sur-Lez, France;cLaboratoire de Parasitologie-Mycologie, Centre Hospitalier Chenevier-Mondor, Assitance Publique-Hopitaux de Paris (APHP), Créteil, France;dUniversité Paris Est-Créteil, UMR BIPAR 956, Créteil, France;eCerba, Cergy-Pontoise, France |
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Abstract: | Invading rodent species can harbor parasites with potential transmission to native rodents and/or humans. To investigate trypanosomes prevalence in rodents, the spleen of 76 rodents from Niger identified by their karyotype was used as a DNA source for Trypanosoma detection using a newly developed qPCR assay. Of the invasive black rat, Rattus rattus, 71% (10/14) were PCR positive as well as 6% (4/62) of native African rodents. Sequences of ∼400 bp of the SSU rDNA gene identified phylogenetically close Trypanosoma lineages. Trypanosoma lewisi was present in all positive black rats and the sequences displayed 100% similarity with T. lewisi-infected humans in Senegal. T. lewisi was also detected in one Acomys johannis, suggesting a possible transmission to native species. In addition to improved knowledge of Trypanosoma diversity in rodents, our data underscore the introduction of the potentially pathogenic T. lewisi kinetoplastid through the human-mediated invasion of black rats all over West Africa. |
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Keywords: | Zoonotic emerging disease Biological invasion Rattus rattus Africa Quantitative real-time PCR |
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