强迫游泳对吗啡依赖小鼠脑内神经递质含量的影响

目的 探讨强迫游泳对吗啡(Mor)精神依赖小鼠脑内单胶类递质的影响。方法 利用条件性位置偏爱(conditioned place preference,CPP)方法，建立小鼠吗啡精神依赖模型；运用HPLC，测定以强迫游泳为刺激因素致小鼠对吗啡侧的偏爱重现时，脑内纹状体、海马、中脑3个部位的NE、DA、5—HT含量的变化。结果 Mor依赖形成(对白箱的偏爱出现)后，纹状体和海马的DA、NE、5—HT及中脑NE的含量均降低；对白箱的偏爱消退后，各部位递质又恢复正常。强迫游泳使海马、纹状体的NE含量大幅度降低，使全部3个部位的DA和5-HT含量升高。结论 脑内神经递质的含量变化与吗啡精神依赖相关。强迫游泳可使小鼠对吗啡侧的偏爱在消退后重现，并对递质含量产生影响，但与Mor依赖对递质含量的影响趋势不完全一致。

Effects of Forced Swimming on Neurotransmitters in Brain of Mice with Psychical Dependence on Morphine

Aim To study the effects of forced swimming on neurotransmitters in brain of mice with psychical dependence on Morphine(Mor).Methods The model of psychical dependence in mice was established with the method of conditioned place preference(CPP).Then,the contents of monoamine transmitters in three regions of brains of mice were determined with HPLC after forced swimming.Results Mor could significantly decrease the contents of NE,DA and 5 HT in the hippocampus and striatum,and that of NE in midbrain.But these changes disappeared when the biasing to white side of the box faded away.Forced swimming could lower the contents of NE in hippocampus and striatum,but promoted the levels of DA and 5 HT acutely in all three regions.Conclusion The contents of neurotransmitters in brain of mice were related with Mor.Forced swimming could reinstate the disappeared Mor CPP in mice,and influenced the contents of neurotransmitters in different trend,but this effect did not accord to that of Mor.