中国汉族和蒙族人群细胞色素氧化酶CYP2C19 遗传多态性的比较

目的：比较中国汉族与蒙族健康人群细胞色素氧化酶CYP2C19遗传多态性。方法：应用限制性片段长度多态性分析法(PCR-RFLP)，对74名汉族健康志愿和6名蒙族健康志愿进行基因多态性分析。结果：蒙族中基因型为野生型纯合子wt／wt的发生率为16．7％(1／6)，汉族中发生率为41．9％(31／74)；蒙族基因型为杂合子wt/ml和wt／m2的为50％(3／6)，汉族中为45．9％(34／74)；蒙族基因型为突变型纯合子m1／m1和m1／m2的为33．3％(2／6)，汉族中为12．2％(9／74)。本实验未发现m2／m2基因型。结论：在中国蒙族和汉族受试中，wt／wt和m1／m1的发生率没有显差别。中国蒙族和汉族健康人群细胞色素氧化酶CYP2C19遗传多态性未显示出统计学差别。

Comparison of genotype polymorphism of cytochrome CYP2C19 between Chinese Han subjects and Chinese Meng subjects

AIM: To compare genotype polymorphism of cytochrome CYP2C19 between Chinese Meng subjects and Chinese Han subjects. METHODS: The genotype polymorphism was analyzed by PCR RFLP (Restriction Fragment Length Polymorphism) in 74 subjects of healthy Chinese Han and 6 subjects of Chinese Meng. RESULTS: Of the 74 genotyped healthy subjects, 31 ( 41.9 %) were homozygous for wildtype (wt/wt), 9 ( 12.2 %) were homozygous for CYP2C19m1 and CYP2C19m2 (m1/m1 or m1/m2 or m2/m2), and 34 ( 45.9 %) were heterozygous for CYP2C19m1 or CYP2C19m2 (m1/wt or m2/wt). Among the 6 genotyped Chinese Meng, 1 ( 16.7 %) was homozygous for wildtype (wt/wt), 2 ( 33.3 %) were homozygous for mutant allele CYP2C19m1 and CYP2C19m2 (m1/m1 or m1/m2 or m2/m2), and 3( 50.0 %) were heterozygous for CYP2C19m1 or CYP2C19m2 (m1/wt or m2/wt); no homozygous genotype for CYP2C19m2(m2/m2)was found in this study. CONCLUSION: There is no statistical difference in occurrence of wt/wt and m1/m1 between in 74 subjects of healthy Chinese Han and 6 subjects of Chinese Meng.