北京市市售花蛤诺如病毒和轮状病毒检出情况及基因特征分析

目的 了解北京市市售花蛤诺如病毒和轮状病毒检出情况,分析其基因特征.方法 2014年11月-2015年10月,采集北京市三个水产品销售市场新鲜花蛤各24件.用两种方法对花蛤鳃和肠内容物进行病毒浓缩,提取核酸后用实时荧光RT-PCR法检测诺如病毒GⅠ/GⅡ组和A组轮状病毒核酸.用半巢式RT-PCR方法,扩增诺如病毒阳性样...

Detection of norovirus and rotavirus and analysis of the genetic characteristics on clams sold in Beijing

Objective To investigate the detection of norovirus and rotavirus and to analyze norovirus genetic characteristics on clams sold in Beijing.Methods Each 24 pieces of fresh clams were collected from A,B and C aquatic products sales markets in Beijing during November 2014 and October 2015.Two method were used to concentrate the virus in gills and intestinal contents of clams.The virus RNA were extracted,real-time RT-PCR was tested for norovirus GI/GII and Rotavirus A.Norovirus capsid protein region was amplified by semi-nested RT-PCR from positive specimens.PCR products were sequenced directly.The subtypes were identified using the norovirus genotyping tool,and the phylogenetic tree was built using MEGA software with maximum likelihood.Results Among 72 sets of scallops,rotavirus was detected negative,and the detection rate of norovirus was 34.7%(25/72).The detection rates in genogroup GII,GI and GI/G II were 27.8%(20/72),4.2%(3/72)and 2.8%(2/72),respectively.The highest detection rate was 100.00%(6/6)in November、The detection rates of norovirus from A,B and C aquatic products sales markets were 25.0%(6/24),50.0%(12/24)and 29.2%(7/24)respectively.6 Strains GII.17 and 4 Strains(GII.6,GII.2,GI.3a and GI.4)were sequenced successfully.The similarity of these strains to diarrhea patients and environmental reference strains were 97.9%-100.0%.Conclusions There is norovirus pollution in clams sold in Beijing,the strains were highly homologous with diarrhea patients and environmental reference strains.The surveillance of norovirus in clams and the population and environment in which they are sold should be strengthened.