| 同源盒基因B2反义寡核苷酸对内皮细胞的影响 |
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| 引用本文： | 张晓启,刘旭盛,刘亮. 同源盒基因B2反义寡核苷酸对内皮细胞的影响[J]. 中华烧伤杂志, 2001, 17(6): 348-350 |
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| 作者姓名： | 张晓启 刘旭盛 刘亮 |
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| 作者单位： | 第三军医大学西南医院全军烧伤研究所 |
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| 基金项目： | 国家重点基础研究发展规划项目资助(G1999054205) |
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| 摘 要： | 目的：探讨同源盒基因B2（HOXB2）反义硫代寡核苷酸对脐静脉内皮细胞增殖，表达的影响。方法：以脂质代介导将硫代磷酸修饰的同源盒基因HOXB2反义寡核苷酸转染入脐静脉内皮细胞，采用MTT和RT－PCR观察不同浓度反义寡核苷酸对内皮细胞增殖及HOXB2 mRNA表达水平的影响，结果：经质体介导的HOXB2反义寡核苷酸转染，内皮细胞增殖受到抑制并呈剂量依赖效应，同时HOXB2 mRNA表达水平显著下降。结论：HOB2在内皮细胞的增殖中起重要作用，提示对HOXB2的研究有可能为创面修复提供新的思路。
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| 关 键 词： | 基因 脐静脉 细胞培养 反义 寡核苷酸类 转染 创面修复术 |
| 修稿时间： | 2001-01-09 |
The influence of HOXB2 anti - sense oligodeoxynucleotides on the proliferation and expression ofhuman umbilical vein endothelial cells |
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| ZHANG Xiaoqi,LIU Xusheng,LIU Liang. The influence of HOXB2 anti - sense oligodeoxynucleotides on the proliferation and expression ofhuman umbilical vein endothelial cells[J]. Chinese journal of burns, 2001, 17(6): 348-350 |
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| Authors: | ZHANG Xiaoqi LIU Xusheng LIU Liang |
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| Affiliation: | Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing 400038, P. R. China. |
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| Abstract: | Objective To explore the effects of HOXB2 anti-sense oligodeoxynucleotides (asodn) on the proliferation and the expression of human umbilical vein endothelial cells (HUVECs). Methods Various concentrations of HOXB2 ASODN modified by thiophosphate were transfected into HUVECs by liposome mediation. MTT and RT-PCR methods were employed to determine the influence of different concentrations of ASODN on endothelial proliferation and the expression level of HOXB2 mRNA. Results After the transfection of HOXB2 ASODN, the endothelial proliferation was inhibited in dose-dependent manner. Simultaneously, the expression level of HOXB2 mRNA decreased significantly. Conclusion HOXB2 might play important roles in the proliferation of endothelial cells. |
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| Keywords: | Gene Primary Human Umbilical Vein Endothelial Cells(HUVECs) Cell Culture Oligodeoxynucleotides Antisense Transfection |
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