21.1.1, a novel activation marker of T and B cells |
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Authors: | V Malard S Amigorena B Bauvois M Daeron M A Cousin D Lando W H Fridman C Sautes |
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Institution: | Laboratoire d'Immunologie, Cellulaire et Clinique, INSERM Unité 255, Institut Curie, Paris, France. |
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Abstract: | A new rat mAb designated mAb 21.1.1 was raised against a T cell hybridoma of mouse origin, T2D4. This antibody, an IgG2b, immunoprecipitates from the membrane extracts of iodinated T2D4 cells a 56-kDa glycoprotein of apparent pI 4.6 which gives a 34-kDa polypeptide after treatment with endoglycosidase F. MAb 21.1.1 reacts with an antigen expressed on murine mitogen-activated thymocytes and T cells, and on B cells stimulated by anti-IgM antibodies. Cells isolated from the spleen, lymph nodes and bone marrow are negative, as are purified resting B cells or T cells. This antigen is strongly expressed on most day-16 fetal thymocytes whereas adult thymocytes are almost negative. mAb 21.1.1 may be useful for the study of activation and differentiation of T and B cells. |
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