贵州洞穴真菌Aspergillus fumigatus GZWMJZ-152的抗菌活性产物

目的 研究贵州梵净山洞穴土壤真菌Aspergillus fumigatus GZWMJZ-152的抗菌活性产物。方法 利用柱色谱及高效液相色谱等对发酵产物进行分离、纯化。运用质谱、核磁共振和比旋光等鉴定化合物的结构。采用药敏纸片法和肉汤稀释法评价化合物的抗菌活性。结果 从烟曲霉GZWMJZ-152的发酵产物中分离并鉴定了9个化合物，其结构分别为烟曲霉酸(1)、gliotoxin(2)、bisdethiobis (methylthio) gliotoxin(3)、fumiquinazoline A(4)、pseurotin A(5)、fumitremorgin C(6)、verruculogen(7)、fumitremorgin B(8)、fumiquinazoline C(9)。化合物1和2对耐甲氧西林金黄色葡萄球菌、副溶血弧菌、蜡样芽孢杆菌、嗜水气单胞菌、假交替单胞菌和黄海希瓦菌具有较强的抑制作用，最低抑菌浓度(minimal inhibitory concentration, MIC)为0.0625~2.0μg/mL。结论 来源于洞穴土壤的真菌能产生具有抗菌活性的次生代谢产物。

Antibacterial natural products from Guizhou cave-derived

Objective To identify the antibacterial secondary metabolites produced by Aspergillus fumigatus GZWMJZ-152, a fungus from a cave in Mount Fanjing in Guizhou. Methods The isolation and purification of compounds were performed by means of column chromatography and HPLC. Their structures were elucidated through the analysis of MS, NMR and specific rotation. The antibacterial activities were assayed by Kirby-Bauer test and the broth dilution method. Results From the cultural broth of Aspergillus fumigatus GZWMJZ-152, nine compounds (1~9) were isolated. Their structures were identified as helvolic acid (1), gliotoxin (2), bisdethiobis (methylthio) gliotoxin (3), fumiquinazoline A (4), pseurotin A (5), fumitremorgin C (6), verruculogen (7), fumitremorgin B (8) and fumiquinazoline C (9), respectively. Compounds 1 and 2 showed strong antibacterial activities against MRSA, Vibrio parahaemolyticus, Bacillus cereus, Aeromonas hydrophila, Pseudoalteromonas nigrifaciens and Shewanella marisflavi with minimal inhibitory concentration values from 0.0625 to 2.0μg/mL. Conclusion The antibacterial natural products can be found from cave