富血小板纤维蛋白应用于年轻恒牙牙髓血运重建术的临床研究

目的：比较富血小板纤维蛋白（platelet-rich fibrin, PRF）牙髓血运重建术和经典牙髓血运重建术对患儿年轻恒牙的临床治疗效果。方法：选择16例患儿的18颗牙髓坏死伴或不伴根尖病变的年轻恒牙，行PRF牙髓血运重建术，选择同期行经典牙髓血运重建术的20例患儿的22颗患牙作为对照组。所有观察患儿／患牙均符合纳入标准，术后定期复查，对其临床表现及X线片进行评估及测量。通过治疗前后的临床记录及影像学资料评价治疗的成功率，并测量和比较治疗前后X线片上患牙的牙根长度、根管壁厚度及根尖孔大小的变化。结果：复查时间6~16个月，两组患儿的大部分患牙症状消失，根尖周病变得到控制或愈合（研究组17/18，对照组21/22），研究组患牙治愈率（83.3%）和对照组患牙治愈率（68.2%）的差异无统计学意义(P=0.446)。影像学结果显示，两组患儿年轻恒牙牙根均有一定程度发育，表现为牙根继续增长，根管壁增厚，根尖孔缩小（研究组15/18，对照组15/22）。研究组患牙根管壁增厚程度较对照组明显（P=0.039）， 两组的牙根长度增加（P=0.411）及根尖孔大小缩小（P=0.737）的差异无统计学意义。在牙髓活力恢复（P=0.517）、根管内钙化(P=0.324)及牙冠变色(P=0.386)方面，两组间差异也无统计学意义。结论：PRF牙髓血运重建术和经典牙髓血运重建术对患儿年轻恒牙的治疗效果均较好，但PRF牙髓血运重建术促进患儿年轻恒牙根管壁增厚的效果优于经典牙髓血运重建术。

Clinical study on autologus platelet-rich fibrin aided revascularization of immature permanent teeth

Objective： To compare the clinical treatment efficiency of platelet-rich fibrin (PRF) assisted revascularization and traditional revascularization in immature permanent teeth. Methods: Eighteen non vital immature permanent teeth of sixteen patients which were diagnosed as necrotic pulpitis with (or without) periapical inflammations were treated with PRF assisted revascularization. Twenty-two teeth non-vital immature permanent teeth of twenty patients which were diagnosed as necrotic pulpitis with (or without) periapical inflammations were treated with traditional revascularization. All the cases were accorded with inclusive criteria and were treated at Pediatric Dentistry of Peking University School and Hospital of Stomatology. The cases were followed up clinically and radiographically at regular intervals. Clinical examinations and periapical radiographs were recorded and analyzed. Clinical success rate was evaluated based on the clinical and radiographic findings. The changes of root length, dentine wall thickness and apical foramen width of the teeth from the two groups were measured and compared according to the preoperative and by recalling the periapical radiographs. Results: The cases were followed up for 6－16 months. Clinically, totally 17 out of the 18 teeth in PRF group and 21 out of the 22 teeth in traditional group were asymptomatic with no sensitivity to percussion or palpation. PRF group tended to be more effective than traditional group clinically without significant differences (P=0.446). Radiographically, 15 out of the 18 teeth in PRF group and 15 out of the 22 teeth in traditional group displayed that the roots developed, showing that root length and dentine wall thickness increased and apical foramen closed. There was a marked difference in dentine wall thickening in PRF group in comparison with traditional group (P=0.039). However, the changes of root length increasing (P=0.411) and apical foramen width closure (P=0.737) were comparable in both groups. The result in electric pulp test (P=0.517), root canal calcification (P=0.324) and crown discolor (P=0.386) were also comparable in the two groups. Conclusion: PRF assisted revascularization and traditional revascularization in non vital immature permanent teeth which were diagnosed as necrotic pulpitis with (or without) periapical inflammations resulted in similar clinical outcomes. Both methods showed good prognosis. PRF revascularization seemed to have better effect on dentine wall thickening than traditional method.