海洋青铜小单孢菌FIM 02-523全基因组测序及分析

青铜小单孢菌(Micromonospora chalcea, M. chalcea)FIM 02-523能够合成对乏氧肿瘤细胞、艰难梭菌等具有活性的环脂肽类化合物rakicidins。利用Illumina HiSeq高通量测序平台，本研究首次对M. chalcea FIM 02-523进行全基因组测序，得到总长约6.74Mb的序列信息。分析表明基因组GC含量为72.89%，包含了6167个蛋白编码序列。利用AntiSMASH预测基因组中存在19个生物合成基因簇。结合PKS/NRPS生物合成特征和rakicidins化学结构特点，定位到了rakicidins的生物合成基因簇，并初步推测其生物合成途径。研究为M. chalcea FIM 02-523的功能基因组学研究和代谢调控提供了理论基础。

Whole-genome sequencing and sequence analysis of marine Micromonospora chalcea FIM 02-523

Micromonospora chalcea (M. chalcea) FIM02-523 is able to synthesize cyclo-lipopeptide rakicidins, which shows hypoxic cytotoxicity and has high activity against Clostridium difficile. In our studies here, the genome sequences of M. chalcea FIM02-523 with a total length of 6.74Mb were first sequenced by the llumina HiSeq sequencing platform. The analysis of genomic data showed that the GC content was 72.98% and the number of coding sequences was 6167. In addition, 19 putative biosynthetic gene clusters were found in the genome through the AntiSMASH gene prediction software. Within these gene clusters, the biosynthetic gene cluster of rakicidins was located by PKS/NRPS biosynthesis specialty and chemical structure. In the end, we proposed the rakicidins biosynthetic pathway. Our study provided a theoretical basis for subsequent future functional genomics studies and further metabolic