高效液相色谱法测定山豆根中红车轴草苷和高丽槐素的含量

目的建立测定山豆根药材中红车轴草苷和高丽槐素含量的HPLC方法。方法色谱柱为Kromasil C18（4.6 mm×200 mm,5μm）,流动相为乙腈-0.1%醋酸溶液,梯度洗脱,流速为1.0 mL.min-1,检测波长为310nm,柱温为25℃。结果红车轴草苷在0.200 6-3.009μg与峰面积线性关系良好,相关系数r=0.999 6,平均回收率为99.6%,RSD=1.2%;高丽槐素在0.305~4.575μg与峰面积线性关系良好,相关系数r=0.999 9,平均回收率为99.2%,RSD=1.1%。结论所用方法简便、准确、重复性好,提供了更好的控制山豆根药材质量的方法。

Determination of trifolirhizin and maackiain in Sophora tonkinensis Gapnep. by HPLC

Objective To establish an HPLC method for the determination of trifolirhizin and maackiain in Sophora tonkinensis Gapnep.Methods The separation was performed on a Kromasi1 C18（4.6 mm×200 mm,5 μm） column.The mobile phase consisted of ACN-0.1%HAc,and gradient elution was used.The flow rate was 1.0 mL·min-1.The UV detective wavelength was 310 nm.Results The calibration curves of trifolirhizin were in good linearity over 0.200 6-3.009 μg（r=0.999 6）.The average recovery was 99.6% with RSD of 1.2%.The calibration curves of maackiain were in good linearity over 0.305-4.575 μg（r=0.999 9）.The average recovery was 99.2% with RSD of 1.1%.Conclusion This method is simple,accurate,repeatable,and provides better quality control for Sophora tonkinensis Gapnep.