| p,p’-DDE对离体培养支持细胞DNA损伤与FasL基因表达的影响 |
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| 引用本文： | 宋杨,杨克敌.p,p’-DDE对离体培养支持细胞DNA损伤与FasL基因表达的影响[J].卫生研究,2006,35(3):261-263. |
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| 作者姓名： | 宋杨 杨克敌 |
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| 作者单位： | 华中科技大学同济医学院公共卫生学院,武汉,430030 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的研究p,p’-DDE对离体培养支持细胞DNA损伤与FasL基因表达的影响。方法从大鼠睾丸组织中分离支持细胞进行离体原代培养3天,加入不同浓度p,p’-DDE继续培养24h,应用单细胞凝胶电泳(SCGE)和反转录聚合链式反应(RT-PCR)研究p,p’-DDE诱导支持细胞DNA损伤和FasL基因表达。结果发现支持细胞DNA迁移度随着p,p’-DDE剂量的增高而增高,同时FasL的基因表达水平也随之增高。结论p,p’-DDE可诱导支持细胞DNA损伤和FasL基因表达,pp’-DDE可能是通过Fas/FasL途径诱导支持细胞损伤,破坏生精过程的动态平衡,最终导致精子减少。
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| 关 键 词： | p p’-DDE 支持细胞 单细胞凝胶电泳 RT-PCRFasL |
| 文章编号： | 1000-8020（2006）03-0261-03 |
| 收稿时间： | 2005-12-30 |
| 修稿时间： | 2005年12月30 |
Effect of p, p'-DDE on DNA damage and expression of FasL gene of rat sertoli cell in vitro |
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| Song Yang,Yang Ke-di.Effect of p, p''''-DDE on DNA damage and expression of FasL gene of rat sertoli cell in vitro[J].Journal of Hygiene Research,2006,35(3):261-263. |
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| Authors: | Song Yang Yang Ke-di |
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| Institution: | Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | ObjectiveTo study the effects of p,p'-DDE on DNA damage and expression of FasL gene of rat sertoli cell in vitro. Methods After separating sertoli cells from testicular tissue of rats,we cultured these cells for 3 days and then added different doses of p,p'-DDE into the culture medium to study its effect on DNA damage and FasL gene expression with the method of SCGE and RT-PCR. Results DNA migration and FasL gene expression of Sertoli cell significantly increased with the increase of the dose of p,p'-DDE. Conclusion p,p'-DDE could induce DNA damage and FasL gene expression of sertoli cells of rat testis. p,p'-DDE might induce the sertoli cell damage and destroy the dynamic process of spermatogenesis through the Fas/FasL pathway, which might lead to oligozoospermatism. |
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| Keywords: | p p'-DDE RT-PCR FasL |
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